Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/8881
Título: | Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger β-galactosidase production |
Autor(es): | Oliveira, Carla Cristina Marques de Teixeira, J. A. Lima, Nelson Silva, Nancy A. da Domingues, Lucília |
Palavras-chave: | Genetic stability of delta-integrating systems Continuous high-cell-density culture Aspergillus niger β-galactosidase production Recombinant Saccharomyces cerevisiae Yeast flocculation Saccharomyces cerevisiae Aspergillus niger beta-galactosidase production recombinant |
Data: | Abr-2007 |
Editora: | The Society for Biotechnology |
Revista: | Journal of Bioscience and Bioengineering |
Citação: | "Journal of Bioscience and Bioengineering." ISSN 1389-1723. 103:4 (Apr. 2007) 318-324. |
Resumo(s): | A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration of the β-galactosidase expression cassette. High-copy-number transformants were successfully obtained using the δ-integration system together with the dominant selection antibiotic, G418. The integration of multiple copies was confirmed by genomic Southern blot analysis. Integrants with the highest β-galactosidase levels (approximately eight gene copies) had similar β-galactosidase activities as a recombinant strain carrying the β-galactosidase expression cassette in a YEp-based vector. The β-galactosidase expression cassettes integrated into the yeast genome were stably maintained after eight sequential batch cultures in a nonselective medium. In continuous high-cell-density culture under the same operating conditions, the integrant strain was more stable than the plasmid-carrying strain. To our knowledge, this is the first study of multicopy δ-integrant stability in a continuous bioreactor operating at different dilution rates. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/8881 |
DOI: | 10.1263/jbb.103.318 |
ISSN: | 1389-1723 |
Versão da editora: | http://www.elsevier.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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OliveiraC_JBB[1].pdf | 362,62 kB | Adobe PDF | Ver/Abrir |