Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/8881
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Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, Carla Cristina Marques de | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Lima, Nelson | - |
dc.contributor.author | Silva, Nancy A. da | - |
dc.contributor.author | Domingues, Lucília | - |
dc.date.accessioned | 2009-04-08T15:52:13Z | - |
dc.date.available | 2009-04-08T15:52:13Z | - |
dc.date.issued | 2007-04 | - |
dc.identifier.citation | "Journal of Bioscience and Bioengineering." ISSN 1389-1723. 103:4 (Apr. 2007) 318-324. | en |
dc.identifier.issn | 1389-1723 | en |
dc.identifier.uri | https://hdl.handle.net/1822/8881 | - |
dc.description.abstract | A flocculent Saccharomyces cerevisiae strain was engineered to stably secrete Aspergillus niger β-galactosidase in a continuous high-cell-density bioreactor. The δ-sequences from the yeast retrotransposon Ty1 were used as target sites for the integration of the β-galactosidase expression cassette. High-copy-number transformants were successfully obtained using the δ-integration system together with the dominant selection antibiotic, G418. The integration of multiple copies was confirmed by genomic Southern blot analysis. Integrants with the highest β-galactosidase levels (approximately eight gene copies) had similar β-galactosidase activities as a recombinant strain carrying the β-galactosidase expression cassette in a YEp-based vector. The β-galactosidase expression cassettes integrated into the yeast genome were stably maintained after eight sequential batch cultures in a nonselective medium. In continuous high-cell-density culture under the same operating conditions, the integrant strain was more stable than the plasmid-carrying strain. To our knowledge, this is the first study of multicopy δ-integrant stability in a continuous bioreactor operating at different dilution rates. | en |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) - Bolsa SFRH/BD/19099/2004 | por |
dc.description.sponsorship | Technical Research Centre (VTT) - Biotechnology, Espoo, Finland. | por |
dc.language.iso | eng | en |
dc.publisher | The Society for Biotechnology | en |
dc.rights | openAccess | en |
dc.subject | Genetic stability of delta-integrating systems | en |
dc.subject | Continuous high-cell-density culture | en |
dc.subject | Aspergillus niger β-galactosidase production | en |
dc.subject | Recombinant Saccharomyces cerevisiae | en |
dc.subject | Yeast flocculation | en |
dc.subject | Saccharomyces cerevisiae | por |
dc.subject | Aspergillus niger beta-galactosidase production | por |
dc.subject | recombinant | por |
dc.title | Development of stable flocculent Saccharomyces cerevisiae strain for continuous Aspergillus niger β-galactosidase production | en |
dc.type | article | por |
dc.peerreviewed | yes | en |
dc.relation.publisherversion | http://www.elsevier.com/ | - |
sdum.number | 4 | en |
sdum.pagination | 318-324 | en |
sdum.publicationstatus | published | en |
sdum.volume | 103 | en |
oaire.citationStartPage | 318 | por |
oaire.citationEndPage | 324 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 103 | por |
dc.identifier.doi | 10.1263/jbb.103.318 | por |
dc.identifier.pmid | 17502272 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Bioscience and Bioengineering | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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OliveiraC_JBB[1].pdf | 362,62 kB | Adobe PDF | Ver/Abrir |