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https://hdl.handle.net/1822/86674
Título: | Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues |
Autor(es): | Moreira, Helena R. Cerqueira, Mariana T. Silva, Lucília Pereira Pires, Joana Jarnalo, Mariana Horta, Ricardo Reis, R. L. Marques, A. P. |
Palavras-chave: | In vitro models Papillary fibroblasts Reticular fibroblasts Skin Vascularization |
Data: | Mai-2023 |
Editora: | Royal Society of Chemistry |
Revista: | Biomaterials Science |
Citação: | Moreira H. R., Cerqueira M. T., da Silva L. P., Pires J., Jarnalo M., Horta R., Reis R. L., Marques A. P. Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues, Biomaterials Science, Vol. 11, pp. 5287, doi:10.1039/D2BM02022J, 2023 |
Resumo(s): | The papillary and reticular dermis harbors phenotypically distinct fibroblasts, whose functions such as maintenance of skin's microvasculature are also distinct. Thus, we hypothesized that pre-selection of the subpopulations of fibroblasts would benefit the generation of skin tissue engineered (TE) constructs, promoting their prevascularization in vitro. We first isolated papillary and reticular fibroblasts using fluorescence-activated cell sorting and studied the effect of their secretome and extracellular matrix (ECM) on human dermal microvascular endothelial cell (hDMEC) organization. Subsequently, we developed a bilayered 3D polymeric structure with distinct layer-associated features to house the subpopulations of fibroblasts, to generate a skin analogue. Both papillary and reticular fibroblasts were able to stimulate capillary-like network formation in a Matrigel assay. However, the secretome of the two subpopulations was substantially different, being enriched in VEGF, IGF-1, and Angio-1 in the case of papillary fibroblasts and in HGF and FGF-2 for the reticular subset. In addition, the fibroblast subpopulations deposited varied levels of ECM proteins, more collagen I and laminin was produced by the reticular subset, but these differences did not impact hDMEC organization. Vessel-like structures with lumens were observed earlier in the 3D skin analogue prepared with the sorted fibroblasts, although ECM deposition was not affected by the cell's pre-selection. Moreover, a more differentiated epidermal layer was obtained in the skin analogue formed by the sorted fibroblasts, confirming that its whole structure was not affected. Overall, we provide evidence that pre-selection of papillary and reticular fibroblasts is relevant for promoting the in vitro prevascularization of skin TE constructs. |
Tipo: | Artigo |
Descrição: | Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2bm02022j |
URI: | https://hdl.handle.net/1822/86674 |
DOI: | 10.1039/D2BM02022J |
ISSN: | 2047-4830 |
e-ISSN: | 2047-4849 |
Versão da editora: | https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm02022j |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20992-pre-selection-fibroblasts-subsets-prompt-prevascularization-tissue-engineered-skin-analogues.pdf | 6,19 MB | Adobe PDF | Ver/Abrir |