Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/86674
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Moreira, Helena R. | por |
dc.contributor.author | Cerqueira, Mariana T. | por |
dc.contributor.author | Silva, Lucília Pereira | por |
dc.contributor.author | Pires, Joana | por |
dc.contributor.author | Jarnalo, Mariana | por |
dc.contributor.author | Horta, Ricardo | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Marques, A. P. | por |
dc.date.accessioned | 2023-10-03T10:33:12Z | - |
dc.date.available | 2023-10-03T10:33:12Z | - |
dc.date.issued | 2023-05 | - |
dc.identifier.citation | Moreira H. R., Cerqueira M. T., da Silva L. P., Pires J., Jarnalo M., Horta R., Reis R. L., Marques A. P. Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues, Biomaterials Science, Vol. 11, pp. 5287, doi:10.1039/D2BM02022J, 2023 | por |
dc.identifier.issn | 2047-4830 | por |
dc.identifier.uri | https://hdl.handle.net/1822/86674 | - |
dc.description | Electronic supplementary information (ESI) available. See DOI: https://doi.org/10.1039/d2bm02022j | - |
dc.description.abstract | The papillary and reticular dermis harbors phenotypically distinct fibroblasts, whose functions such as maintenance of skin's microvasculature are also distinct. Thus, we hypothesized that pre-selection of the subpopulations of fibroblasts would benefit the generation of skin tissue engineered (TE) constructs, promoting their prevascularization in vitro. We first isolated papillary and reticular fibroblasts using fluorescence-activated cell sorting and studied the effect of their secretome and extracellular matrix (ECM) on human dermal microvascular endothelial cell (hDMEC) organization. Subsequently, we developed a bilayered 3D polymeric structure with distinct layer-associated features to house the subpopulations of fibroblasts, to generate a skin analogue. Both papillary and reticular fibroblasts were able to stimulate capillary-like network formation in a Matrigel assay. However, the secretome of the two subpopulations was substantially different, being enriched in VEGF, IGF-1, and Angio-1 in the case of papillary fibroblasts and in HGF and FGF-2 for the reticular subset. In addition, the fibroblast subpopulations deposited varied levels of ECM proteins, more collagen I and laminin was produced by the reticular subset, but these differences did not impact hDMEC organization. Vessel-like structures with lumens were observed earlier in the 3D skin analogue prepared with the sorted fibroblasts, although ECM deposition was not affected by the cell's pre-selection. Moreover, a more differentiated epidermal layer was obtained in the skin analogue formed by the sorted fibroblasts, confirming that its whole structure was not affected. Overall, we provide evidence that pre-selection of papillary and reticular fibroblasts is relevant for promoting the in vitro prevascularization of skin TE constructs. | por |
dc.description.sponsorship | The authors would like to acknowledge the financial support from the Consolidator Grant “ECM_INK” (ERC-2016- COG-726061), to the FSE/POCH (Fundo Social Europeu através do Programa Operacional do Capital Humano) under the scope of the PD/169/2013, NORTE-08-5369-FSE-000037 (H.R. M.). | por |
dc.language.iso | eng | por |
dc.publisher | Royal Society of Chemistry | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/726061/EU | por |
dc.relation | PD/169/2013 | por |
dc.rights | openAccess | por |
dc.subject | In vitro models | por |
dc.subject | Papillary fibroblasts | por |
dc.subject | Reticular fibroblasts | por |
dc.subject | Skin | por |
dc.subject | Vascularization | por |
dc.title | Pre-selection of fibroblasts subsets prompt prevascularization of tissue engineered skin analogues | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.rsc.org/en/content/articlelanding/2023/bm/d2bm02022j | por |
dc.comments | http://3bs.uminho.pt/node/20992 | por |
oaire.citationStartPage | 5287 | por |
oaire.citationEndPage | 5300 | por |
oaire.citationIssue | 15 | por |
oaire.citationVolume | 11 | por |
dc.date.updated | 2023-10-03T09:25:01Z | - |
dc.identifier.eissn | 2047-4849 | por |
dc.identifier.doi | 10.1039/D2BM02022J | por |
dc.identifier.pmid | 37350513 | por |
sdum.journal | Biomaterials Science | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
20992-pre-selection-fibroblasts-subsets-prompt-prevascularization-tissue-engineered-skin-analogues.pdf | 6,19 MB | Adobe PDF | Ver/Abrir |