Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/85172
Título: | StressMatic: a novel automated system to induce depressive- and anxiety-like phenotype in rats |
Autor(es): | Martins-Macedo, Joana Mateus-Pinheiro, António Alves, Cátia Veloso, Fernando José Silva Gomes, Eduardo D. Ribeiro, Inês Correia, Joana S. Silveira-Rosa, Tiago Alves, Nuno Dinis Lopes Oliveira Rodrigues, Ana J. Bessa, João M. Sousa, Nuno Oliveira, João F. Patrício, Patrícia Pinto, Luísa |
Palavras-chave: | Automated rack Stress-exposure Protocols standardization Preclinical research |
Data: | 20-Jan-2023 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Cells |
Citação: | Martins-Macedo, J.; Mateus-Pinheiro, A.; Alves, C.; Veloso, F.; Gomes, E.D.; Ribeiro, I.; Correia, J.S.; Silveira-Rosa, T.; Alves, N.D.; Rodrigues, A.J.; et al. StressMatic: A Novel Automated System to Induce Depressive- and Anxiety-like Phenotype in Rats. Cells 2023, 12, 381. https://doi.org/10.3390/cells12030381 |
Resumo(s): | Major depressive disorder (MDD) is a multidimensional psychiatric disorder that is estimated to affect around 350 million people worldwide. Generating valid and effective animal models of depression is critical and has been challenging for neuroscience researchers. For preclinical studies, models based on stress exposure, such as unpredictable chronic mild stress (uCMS), are amongst the most reliable and used, despite presenting concerns related to the standardization of protocols and time consumption for operators. To overcome these issues, we developed an automated system to expose rodents to a standard uCMS protocol. Here, we compared manual (uCMS) and automated (auCMS) stress-exposure protocols. The data shows that the impact of the uCMS exposure by both methods was similar in terms of behavioral (cognition, mood, and anxiety) and physiological (cell proliferation and endocrine variations) measurements. Given the advantages of time and standardization, this automated method represents a step forward in this field of preclinical research. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/85172 |
DOI: | 10.3390/cells12030381 |
e-ISSN: | 2073-4409 |
Versão da editora: | https://www.mdpi.com/2073-4409/12/3/381 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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cells-12-00381.pdf | 4,06 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons