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https://hdl.handle.net/1822/79692
Título: | Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases |
Autor(es): | Vasconcelos, Filipa Lima, Ana C. Bonani, Walter Silva, Catarina S. Reis, R. L. Motta, Antonella Migliaresi, Claudio Martins, Albino Neves, N. M. |
Palavras-chave: | Anti-TNFα antibody Coaxial electrospinning Inflammatory arthritic diseases Methotrexate Microfluidic-assisted electrospinning |
Data: | Fev-2022 |
Editora: | Elsevier 1 |
Revista: | Biomaterials Advances |
Citação: | Vasconcelos F., Lima A. C., Bonani W., Silva C. S., Reis R. L., Motta A., Migliaresi C., Martins A., Neves N. M. Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases, Biomaterials Advances, Vol. 134, pp. 112585, doi:10.1016/j.msec.2021.112585, 2022 |
Resumo(s): | Inflammatory arthritic diseases are characterized by a persistent inflammation of the synovial tissues where tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) pro-inflammatory cytokines are over-expressed, leading to progressive musculoskeletal disability. Methotrexate (MTX), a disease-modifying-anti-rheumatic drug (DMARD) commonly applied in their treatment, can be used in combination with biological-DMARDs as anti-TNFα antibody to improve the treatments efficacy. However, their systemic administration comes with severe side-effects and limited therapeutic efficacy due to their off-target distribution and short half-life. To overcome such limitations, encapsulation of clinically relevant concentrations of MTX and anti-TNFα antibody into polycaprolactone (PCL) or poly(vinyl-alcohol) (PVA) microfluidic-assisted or coaxial electrospun fibrous meshes is proposed as local controlled dual drug release systems. Release studies show that microfluidic-assisted electrospinning meshes encapsulating both drugs achieved higher concentrations than coaxials. Biological assays using human articular chondrocytes (hACs) and monocytic cells (THP-1 cell line) demonstrate that fibrous meshes encapsulating the drugs are non-toxic. The systems' efficacy is proved by a significant decrease of TNFα and IL-6 concentrations in conditioned medium of lipopolysaccharide (LPS)-stimulated THP-1 cells, especially in the presence of microfluidic-assisted electrospun meshes, when compared with THP-1 conditioned medium (59.5% and 83.9% less, respectively). Therefore, microfluidic-assisted electrospinning fibrous meshes with encapsulating drugs represent an alternative to coaxial, as a local therapy for inflammatory arthritis diseases. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/79692 |
DOI: | 10.1016/j.msec.2021.112585 |
ISSN: | 0928-4931 |
e-ISSN: | 2772-9508 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0928493121007256 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20721-microfluidic-assisted-electrospinning-alternative-coaxial-controlled-dual-drug-release-system-treat-.pdf | 4,87 MB | Adobe PDF | Ver/Abrir |