Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/79692
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Campo DC | Valor | Idioma |
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dc.contributor.author | Vasconcelos, Filipa | por |
dc.contributor.author | Lima, Ana C. | por |
dc.contributor.author | Bonani, Walter | por |
dc.contributor.author | Silva, Catarina S. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Motta, Antonella | por |
dc.contributor.author | Migliaresi, Claudio | por |
dc.contributor.author | Martins, Albino | por |
dc.contributor.author | Neves, N. M. | por |
dc.date.accessioned | 2022-09-26T11:03:06Z | - |
dc.date.available | 2022-09-26T11:03:06Z | - |
dc.date.issued | 2022-02 | - |
dc.identifier.citation | Vasconcelos F., Lima A. C., Bonani W., Silva C. S., Reis R. L., Motta A., Migliaresi C., Martins A., Neves N. M. Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases, Biomaterials Advances, Vol. 134, pp. 112585, doi:10.1016/j.msec.2021.112585, 2022 | por |
dc.identifier.issn | 0928-4931 | por |
dc.identifier.uri | https://hdl.handle.net/1822/79692 | - |
dc.description.abstract | Inflammatory arthritic diseases are characterized by a persistent inflammation of the synovial tissues where tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) pro-inflammatory cytokines are over-expressed, leading to progressive musculoskeletal disability. Methotrexate (MTX), a disease-modifying-anti-rheumatic drug (DMARD) commonly applied in their treatment, can be used in combination with biological-DMARDs as anti-TNFα antibody to improve the treatments efficacy. However, their systemic administration comes with severe side-effects and limited therapeutic efficacy due to their off-target distribution and short half-life. To overcome such limitations, encapsulation of clinically relevant concentrations of MTX and anti-TNFα antibody into polycaprolactone (PCL) or poly(vinyl-alcohol) (PVA) microfluidic-assisted or coaxial electrospun fibrous meshes is proposed as local controlled dual drug release systems. Release studies show that microfluidic-assisted electrospinning meshes encapsulating both drugs achieved higher concentrations than coaxials. Biological assays using human articular chondrocytes (hACs) and monocytic cells (THP-1 cell line) demonstrate that fibrous meshes encapsulating the drugs are non-toxic. The systems' efficacy is proved by a significant decrease of TNFα and IL-6 concentrations in conditioned medium of lipopolysaccharide (LPS)-stimulated THP-1 cells, especially in the presence of microfluidic-assisted electrospun meshes, when compared with THP-1 conditioned medium (59.5% and 83.9% less, respectively). Therefore, microfluidic-assisted electrospinning fibrous meshes with encapsulating drugs represent an alternative to coaxial, as a local therapy for inflammatory arthritis diseases. | por |
dc.description.sponsorship | This work was supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, for the Ph.D grant of Catarina Silva (UMINHO/BD/33/2016; NORTE-08-5369-FSE-000012), and by the Portuguese Science and Technology Foundation (FCT) for the cells project Cells4_ID (PTDC/BTM-SAL/28882/2017). | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | UMINHO/BD/33/2016 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FBTM-SAL%2F28882%2F2017/PT | por |
dc.rights | openAccess | por |
dc.subject | Anti-TNFα antibody | por |
dc.subject | Coaxial electrospinning | por |
dc.subject | Inflammatory arthritic diseases | por |
dc.subject | Methotrexate | por |
dc.subject | Microfluidic-assisted electrospinning | por |
dc.title | Microfluidic-assisted electrospinning, an alternative to coaxial, as a controlled dual drug release system to treat inflammatory arthritic diseases | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0928493121007256 | por |
dc.comments | http://3bs.uminho.pt/node/20721 | por |
oaire.citationVolume | 134 | por |
dc.date.updated | 2022-09-26T09:48:43Z | - |
dc.identifier.eissn | 2772-9508 | por |
dc.identifier.doi | 10.1016/j.msec.2021.112585 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biomaterials Advances | por |
dc.identifier.articlenumber | 112585 | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20721-microfluidic-assisted-electrospinning-alternative-coaxial-controlled-dual-drug-release-system-treat-.pdf | 4,87 MB | Adobe PDF | Ver/Abrir |