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https://hdl.handle.net/1822/75597
Título: | Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates |
Autor(es): | Terra-Matos, Joana Teixeira, Marta Oliveira Pereira, Cátia Sofia Santos Noronha, Henrique Domingues, Lucília Sieiro, Carmen Gerós, H. Chaves, Susana Alexandra Rodrigues Sousa, Maria João Côrte-Real, Manuela |
Palavras-chave: | Saccharomyces cerevisiae Acetic acid Lignocellulosic hydrolysates Vacuolar zinc transporter Lignocellulosic ethanol |
Data: | 14-Jan-2022 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Journal of Fungi |
Citação: | Terra-Matos, Joana; Teixeira, Marta Oliveira; Cátia S. Pereira; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, Hernâni; Chaves, Susana Rodrigues; Sousa, Maria João; Côrte-Real, Manuela, Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates. Journal of Fungi, 8(1), 78, 2022 |
Resumo(s): | Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/75597 |
DOI: | 10.3390/jof8010078 |
ISSN: | 2309-608X |
Versão da editora: | http://www.mdpi.com/journal/jof |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55169_1.pdf | 2,93 MB | Adobe PDF | Ver/Abrir |