Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/75597
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Terra-Matos, Joana | por |
dc.contributor.author | Teixeira, Marta Oliveira | por |
dc.contributor.author | Pereira, Cátia Sofia Santos | por |
dc.contributor.author | Noronha, Henrique | por |
dc.contributor.author | Domingues, Lucília | por |
dc.contributor.author | Sieiro, Carmen | por |
dc.contributor.author | Gerós, H. | por |
dc.contributor.author | Chaves, Susana Alexandra Rodrigues | por |
dc.contributor.author | Sousa, Maria João | por |
dc.contributor.author | Côrte-Real, Manuela | por |
dc.date.accessioned | 2022-01-20T09:34:21Z | - |
dc.date.available | 2022-01-20T09:34:21Z | - |
dc.date.issued | 2022-01-14 | - |
dc.identifier.citation | Terra-Matos, Joana; Teixeira, Marta Oliveira; Cátia S. Pereira; Noronha, Henrique; Domingues, Lucília; Sieiro, Carmen; Gerós, Hernâni; Chaves, Susana Rodrigues; Sousa, Maria João; Côrte-Real, Manuela, Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates. Journal of Fungi, 8(1), 78, 2022 | por |
dc.identifier.issn | 2309-608X | por |
dc.identifier.uri | https://hdl.handle.net/1822/75597 | - |
dc.description.abstract | Yeast-based bioethanol production from lignocellulosic hydrolysates (LH) is an attractive and sustainable alternative for biofuel production. However, the presence of acetic acid (AA) in LH is still a major problem. Indeed, above certain concentrations, AA inhibits yeast fermentation and triggers a regulated cell death (RCD) process mediated by the mitochondria and vacuole. Understanding the mechanisms involved in AA-induced RCD (AA-RCD) may thus help select robust fermentative yeast strains, providing novel insights to improve lignocellulosic ethanol (LE) production. Herein, we hypothesized that zinc vacuolar transporters are involved in vacuole-mediated AA-RCD, since zinc enhances ethanol production and zinc-dependent catalase and superoxide dismutase protect from AA-RCD. In this work, zinc limitation sensitized wild-type cells to AA-RCD, while zinc supplementation resulted in a small protective effect. Cells lacking the vacuolar zinc transporter Zrt3 were highly resistant to AA-RCD, exhibiting reduced vacuolar dysfunction. Moreover, zrt3Δ cells displayed higher ethanol productivity than their wild-type counterparts, both when cultivated in rich medium with AA (0.29 g L−1 h−1 versus 0.11 g L−1 h−1) and in an LH (0.73 g L−1 h−1 versus 0.55 g L−1 h−1). Overall, the deletion of ZRT3 emerges as a promising strategy to increase strain robustness in LE industrial production. | por |
dc.description.sponsorship | This work was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of “Contrato-Programa” UIDB/04050/2020 - PE 20-23 and UIDB/04469/2020 unit. Cátia Santos-Pereira acknowledges the Ph.D. fellowship PD/BD/128032/2016 funded by FCT under the scope of the doctoral program in Applied and Environmental Microbiology (DP_AEM). | por |
dc.description.sponsorship | The authors acknowledge Helena Pereira for constructing the pRS413-Pep4- mCherry plasmid and Sara L. Baptista for the preparation of vine prune residue autohydrolysis liquors. BIOVINO project (0688_BIOVINO_6_E) funded by INTERREG España—Portugal and European Regional Development Fund (ERDF) is acknowledged for the supply of vine prune residues | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute (MDPI) | por |
dc.relation | UIDB/04050/2020 | por |
dc.relation | UIDB/04469/2020 | por |
dc.relation | PD/BD/128032/2016 | por |
dc.rights | openAccess | por |
dc.subject | Saccharomyces cerevisiae | por |
dc.subject | Acetic acid | por |
dc.subject | Lignocellulosic hydrolysates | por |
dc.subject | Vacuolar zinc transporter | por |
dc.subject | Lignocellulosic ethanol | por |
dc.title | Saccharomyces cerevisiae cells lacking the zinc vacuolar transporter Zrt3 display improved ethanol productivity in lignocellulosic hydrolysates | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/jof | por |
dc.comments | CEB55169 | por |
oaire.citationStartPage | 78 | por |
oaire.citationIssue | 1 | por |
oaire.citationConferencePlace | Switzerland | - |
oaire.citationVolume | 8 | por |
dc.date.updated | 2022-01-19T22:28:01Z | - |
dc.identifier.doi | 10.3390/jof8010078 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Fungi | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_55169_1.pdf | 2,93 MB | Adobe PDF | Ver/Abrir |