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https://hdl.handle.net/1822/74385
Título: | Epilactose biosynthesis using recombinant cellobiose 2-epimerase produced by Saccharomyces cerevisiae |
Autor(es): | Cardoso, Beatriz Alexandra Batista Silvério, Sara Isabel Cruz Rodrigues, Joana Lúcia Lima Correia Rodrigues, L. R. |
Palavras-chave: | Fungi Peptides and proteins Bacteria Genetics Dietary fiber Saccharomyces cerevisiae Cellobiose 2-epimerase Lactose-based prebiotics Caldicellulosiruptor saccharolyticus epilactose |
Data: | 10-Set-2021 |
Editora: | American Chemical Society |
Revista: | Food Science and Technology |
Citação: | Cardoso, B.; Silvério, Sara C.; Rodrigues, Joana L.; Rodrigues, Lígia R., Epilactose biosynthesis using recombinant cellobiose 2-epimerase produced by Saccharomyces cerevisiae. ACS Food Science & Technology, 1(9), 1578-1584, 2021 |
Resumo(s): | Cellobiose 2-epimerases are interesting enzymes that can catalyze the production of lactose-based prebiotics, namely, epilactose and lactulose, through a single substrate reaction with noteworthy yields. In this work, the GRAS yeast Saccharomyces cerevisiae was for the first time used as a host for the heterologous production of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus. The enzyme showed an optimal temperature and an optimal pH of 80 °C and 7.5, respectively. Under these conditions, the time-course production of epilactose from lactose (50 g/L) was evaluated. The maximum yield and productivity obtained were 27\% and 9 g L1 h1, respectively. This epilactose yield represents the highest value obtained from the several approaches reported using cellobiose 2-epimerase from C. saccharolyticus. In conclusion, it was shown that the enzyme produced by S. cerevisiae is suitable for the production of epilactose-enriched prebiotic mixtures with potential applications in the food industry. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/74385 |
DOI: | 10.1021/acsfoodscitech.1c00183 |
ISSN: | 2692-1944 |
Versão da editora: | https://pubs.acs.org/doi/abs/10.1021/acsfoodscitech.1c00183 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
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document_54630_1.pdf Acesso restrito! | 306,35 kB | Adobe PDF | Ver/Abrir |