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https://hdl.handle.net/1822/67635
Título: | In-vivo in situ en-face optical coherence tomography imaging of chick embryos |
Autor(es): | Leitner, Michael Castanheira, Joana Ferreira, Luís Ferreira, Mónica Palmeirim, Isabel Rosa, Carla C. Podoleanu, Adrian Gh. |
Palavras-chave: | Optical coherence tomography Time-domain OCT en-face OCT Development biology Chick embryos Invivo imaging |
Data: | 2009 |
Editora: | International Society For Optics And Photonics (SPIE) |
Revista: | Proceedings of SPIE-OSA Biomedical Optics |
Resumo(s): | Chick embryos are among the most studied species in development biology because they are easily obtained, highly accessible and present a similar development to that of humans. Normally morphological studies are carried out with confocal microscopy, however in-situ imaging is impossible and in- vivo imaging can only be performed with great difficulty. For confocal microscopy the embryo has to be studied outside the egg, what generally also means a short life expectancy of the embryo. Additionally, extracting the embryo of the egg precludes the possibility of studying its development in its natural environment. In this paper it is shown that en-face optical coherence tomography (en-face OCT) is a possible solution to overcome these difficulties allowing for an in-situ and in-vivo study over a timescale of several days. With en-face OCT it is possible to accompany the development of one single embryo over several days and to acquire high resolution and axially resolved images. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/67635 |
ISBN: | 9780819476487 |
DOI: | 10.1117/12.831859 |
ISSN: | 1605-7422 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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leitner2009.pdf Acesso restrito! | 610,33 kB | Adobe PDF | Ver/Abrir |