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https://hdl.handle.net/1822/67635
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Campo DC | Valor | Idioma |
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dc.contributor.author | Leitner, Michael | por |
dc.contributor.author | Castanheira, Joana | por |
dc.contributor.author | Ferreira, Luís | por |
dc.contributor.author | Ferreira, Mónica | por |
dc.contributor.author | Palmeirim, Isabel | por |
dc.contributor.author | Rosa, Carla C. | por |
dc.contributor.author | Podoleanu, Adrian Gh. | por |
dc.date.accessioned | 2020-10-22T12:28:39Z | - |
dc.date.issued | 2009 | - |
dc.identifier.isbn | 9780819476487 | - |
dc.identifier.issn | 1605-7422 | - |
dc.identifier.uri | https://hdl.handle.net/1822/67635 | - |
dc.description.abstract | Chick embryos are among the most studied species in development biology because they are easily obtained, highly accessible and present a similar development to that of humans. Normally morphological studies are carried out with confocal microscopy, however in-situ imaging is impossible and in- vivo imaging can only be performed with great difficulty. For confocal microscopy the embryo has to be studied outside the egg, what generally also means a short life expectancy of the embryo. Additionally, extracting the embryo of the egg precludes the possibility of studying its development in its natural environment. In this paper it is shown that en-face optical coherence tomography (en-face OCT) is a possible solution to overcome these difficulties allowing for an in-situ and in-vivo study over a timescale of several days. With en-face OCT it is possible to accompany the development of one single embryo over several days and to acquire high resolution and axially resolved images. | por |
dc.description.sponsorship | Michael Leitner acknowledges the support of the Marie Curie Early Stage Researcher training network “High resolution optical measurement and imaging”, sponsored by the European Commission MEST-CT-2005-020353, at the Faculdade de Ciências of University of Porto, Porto, Portugal and the Applied Optics Group, School of Physical Sciences at University of Kent at Canterbury, Canterbury, UK. | por |
dc.language.iso | eng | por |
dc.publisher | International Society For Optics And Photonics (SPIE) | por |
dc.rights | closedAccess | por |
dc.subject | Optical coherence tomography | por |
dc.subject | Time-domain OCT | por |
dc.subject | en-face OCT | por |
dc.subject | Development biology | por |
dc.subject | Chick embryos | por |
dc.subject | Invivo imaging | por |
dc.title | In-vivo in situ en-face optical coherence tomography imaging of chick embryos | por |
dc.type | conferencePaper | por |
dc.peerreviewed | yes | por |
oaire.citationStartPage | 73720Y-1 | por |
oaire.citationEndPage | 73720Y-7 | por |
oaire.citationVolume | 7372 | por |
dc.identifier.doi | 10.1117/12.831859 | por |
dc.date.embargo | 10000-01-01 | - |
sdum.journal | Proceedings of SPIE-OSA Biomedical Optics | por |
sdum.conferencePublication | Optical Coherence Tomography and Coherence Techniques IV | por |
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leitner2009.pdf Acesso restrito! | 610,33 kB | Adobe PDF | Ver/Abrir |