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https://hdl.handle.net/1822/67326
Título: | Retinoic acid as a modulator of proximal-distal patterning and branching morphogenesis of the avian lung |
Autor(es): | Moura, Rute S. |
Palavras-chave: | Animals Body Patterning Chick Embryo Gene Expression Regulation, Developmental In Situ Hybridization Lung branching Morphogenesis Organ Culture Techniques SOX9 Transcription Factor SOXB1 Transcription Factors Tretinoin Proximal-distal patterning Explant culture In situ hybridization Morphometric analysis Chicken embryo |
Data: | 2019 |
Editora: | Humana Press |
Revista: | Methods in Molecular Biology |
Citação: | Moura, R.S. (2019). Retinoic Acid as a Modulator of Proximal-Distal Patterning and Branching Morphogenesis of the Avian Lung. In: Ray, S. (eds) Retinoid and Rexinoid Signaling . Methods in Molecular Biology, vol 2019. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9585-1_15 |
Resumo(s): | Retinoic acid modulates numerous cellular events, namely, proliferation, differentiation, apoptosis, and patterning, hence influencing both embryo development and adult homeostasis. In vitro explant culture is a valuable technique for studying the impact of growth factors and signaling molecules, such as retinoic acid, in organ development since tissue architecture is maintained. This technique allows controlled supplementation of culture medium and straightforward analysis of its effect on morphogenesis. This chapter describes the detailed protocol for culturing embryonic chick lung explants and testing the impact of retinoic acid in branching and patterning, based on morphometric and molecular analysis. |
Tipo: | Capítulo de livro |
Descrição: | First Online: 30 July 2019 |
URI: | https://hdl.handle.net/1822/67326 |
ISBN: | 978-1-4939-9584-4 |
e-ISBN: | 978-1-4939-9585-1 |
DOI: | 10.1007/978-1-4939-9585-1_15 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/protocol/10.1007/978-1-4939-9585-1_15 |
Acesso: | Acesso restrito UMinho |
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10.1007@978-1-4939-9585-115.pdf Acesso restrito! | 360,94 kB | Adobe PDF | Ver/Abrir |