Retinoic acid as a modulator of proximal-distal patterning and branching morphogenesis of the avian lung

doi:10.1007/978-1-4939-9585-1_15

Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/67326

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Campo DC	Valor	Idioma
dc.contributor.author	Moura, Rute S.	por
dc.date.accessioned	2020-10-07T11:00:35Z	-
dc.date.issued	2019	-
dc.identifier.citation	Moura, R.S. (2019). Retinoic Acid as a Modulator of Proximal-Distal Patterning and Branching Morphogenesis of the Avian Lung. In: Ray, S. (eds) Retinoid and Rexinoid Signaling . Methods in Molecular Biology, vol 2019. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9585-1_15	por
dc.identifier.isbn	978-1-4939-9584-4	por
dc.identifier.issn	1064-3745	por
dc.identifier.uri	https://hdl.handle.net/1822/67326	-
dc.description	First Online: 30 July 2019	-
dc.description.abstract	Retinoic acid modulates numerous cellular events, namely, proliferation, differentiation, apoptosis, and patterning, hence influencing both embryo development and adult homeostasis. In vitro explant culture is a valuable technique for studying the impact of growth factors and signaling molecules, such as retinoic acid, in organ development since tissue architecture is maintained. This technique allows controlled supplementation of culture medium and straightforward analysis of its effect on morphogenesis. This chapter describes the detailed protocol for culturing embryonic chick lung explants and testing the impact of retinoic acid in branching and patterning, based on morphometric and molecular analysis.	por
dc.description.sponsorship	FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the Project UID/Multi/50026/2019; and by the Project NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER)	por
dc.language.iso	eng	por
dc.publisher	Humana Press	por
dc.relation	info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UID%2FMulti%2F50026%2F2019/PT	-
dc.rights	restrictedAccess	por
dc.subject	Animals	por
dc.subject	Body Patterning	por
dc.subject	Chick Embryo	por
dc.subject	Gene Expression Regulation, Developmental	por
dc.subject	In Situ Hybridization	por
dc.subject	Lung branching	por
dc.subject	Morphogenesis	por
dc.subject	Organ Culture Techniques	por
dc.subject	SOX9 Transcription Factor	por
dc.subject	SOXB1 Transcription Factors	por
dc.subject	Tretinoin	por
dc.subject	Proximal-distal patterning	por
dc.subject	Explant culture	por
dc.subject	In situ hybridization	por
dc.subject	Morphometric analysis	por
dc.subject	Chicken embryo	por
dc.title	Retinoic acid as a modulator of proximal-distal patterning and branching morphogenesis of the avian lung	por
dc.type	bookPart	por
dc.relation.publisherversion	https://link.springer.com/protocol/10.1007/978-1-4939-9585-1_15	por
oaire.citationStartPage	209	por
oaire.citationEndPage	224	por
oaire.citationVolume	2019	por
dc.identifier.doi	10.1007/978-1-4939-9585-1_15	por
dc.date.embargo	10000-01-01	-
dc.identifier.eisbn	978-1-4939-9585-1	por
dc.identifier.pmid	31359399	por
dc.subject.fos	Ciências Médicas::Medicina Básica	por
dc.subject.wos	Science & Technology	por
sdum.journal	Methods in Molecular Biology	-
sdum.conferencePublication	RETINOID AND REXINOID SIGNALING: METHODS AND PROTOCOLS	por
Aparece nas coleções:	ICVS - Livros e capítulos de Livros / Books and book chapters