Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61335
Título: | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains |
Autor(es): | Díaz-Fernández, David Aguiar, Tatiana Quinta Martín, Victoria Isabel Romani, Aloia Perez Silva, Rui Domingues, Lucília Revuelta, José Luis Jiménez, Alberto |
Palavras-chave: | Ashbya gossypii microbial lipids regulation lignocellulosic hydrolysates xylose molasses glycerol |
Data: | Dez-2019 |
Editora: | Elsevier 1 |
Revista: | Bioresource Technology |
Citação: | Díaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 2019 |
Resumo(s): | This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks. |
Tipo: | Artigo |
Descrição: | Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054. |
URI: | https://hdl.handle.net/1822/61335 |
DOI: | 10.1016/j.biortech.2019.122054 |
ISSN: | 0960-8524 |
e-ISSN: | 0960-8524 |
Versão da editora: | http://www.journals.elsevier.com/bioresource-technology/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51947_1.pdf | 1,18 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons