Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61335
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Díaz-Fernández, David | por |
dc.contributor.author | Aguiar, Tatiana Quinta | por |
dc.contributor.author | Martín, Victoria Isabel | por |
dc.contributor.author | Romani, Aloia Perez | por |
dc.contributor.author | Silva, Rui | por |
dc.contributor.author | Domingues, Lucília | por |
dc.contributor.author | Revuelta, José Luis | por |
dc.contributor.author | Jiménez, Alberto | por |
dc.date.accessioned | 2019-09-09T08:14:48Z | - |
dc.date.available | 2019-09-09T08:14:48Z | - |
dc.date.issued | 2019-12 | - |
dc.identifier.citation | Díaz-Fernández, David; Aguiar, Tatiana Q.; Martín, Victoria Isabel; Romaní, Aloia; Silva, Rui; Domingues, Lucília; Revuelta, José Luis; Jiménez, Alberto, Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains. Bioresource Technology, 293(122054), 2019 | por |
dc.identifier.issn | 0960-8524 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61335 | - |
dc.description | Supplementary data to this article can be found online at https:// doi.org/10.1016/j.biortech.2019.122054. | por |
dc.description.abstract | This work presents the exploitation of waste industrial by-products as raw materials for the production of microbial lipids in engineered strains of the filamentous fungus Ashbya gossypii. A lipogenic xylose-utilizing strain was used to apply a metabolic engineering approach aiming at relieving regulatory mechanisms to further increase the biosynthesis of lipids. Three genomic manipulations were applied: the overexpression of a feedback resistant form of the acetyl-CoA carboxylase enzyme; the expression of a truncated form of Mga2, a regulator of the main 9 desaturase gene; and the overexpression of an additional copy of DGA1 that codes for diacylglycerol acyltransferase. The performance of the engineered strain was evaluated in culture media containing mixed formulations of corn-cob hydrolysates, sugarcane molasses or crude glycerol. Our results demonstrate the efficiency of the engineered strains, which were able to accumulate about 40% of cell dry weight (CDW) in lipid content using organic industrial wastes as feedstocks. | por |
dc.description.sponsorship | This work was supported by: the Spanish Ministerio de Economía y Competitividad (grant numbers BIO2014-56930-PandBIO2017-88435R) and the Junta de Castilla y León (grant number SA016P17); the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019, PhD grant PD/ BD/113812/2015 to R. Silva (Doctoral Program in Applied and Environmental Microbiology), the MultiBiorefinery project (POCI-010145-FEDER-016403) and the BioTecNorte operation (NORTE-010145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. DDF was recipient of USAL predoctoral fellowship from the University of Salamanca. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.relation | PD/BD/113812/2015 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by-nd/4.0/ | por |
dc.subject | Ashbya gossypii | por |
dc.subject | microbial lipids | por |
dc.subject | regulation | por |
dc.subject | lignocellulosic hydrolysates | por |
dc.subject | xylose | por |
dc.subject | molasses | por |
dc.subject | glycerol | por |
dc.title | Microbial lipids from industrial wastes using xylose-utilizing Ashbya gossypii strains | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/bioresource-technology/ | por |
dc.comments | CEB51947 | por |
oaire.citationVolume | 293 | por |
dc.date.updated | 2019-09-08T09:57:40Z | - |
dc.identifier.eissn | 0960-8524 | por |
dc.identifier.doi | 10.1016/j.biortech.2019.122054 | por |
dc.identifier.pmid | 31487616 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Bioresource Technology | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51947_1.pdf | 1,18 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons