Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61259
Título: | Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains |
Autor(es): | Rodriguez, R. Santos, Carla Simões, Marta Filipa Soares, Célia Santos, Cledir Lima, Nelson |
Palavras-chave: | freeze-drying accelerated ageing mass spectrometry DNA fingerprinting biotechnological potential |
Data: | 2019 |
Editora: | MDPI Publishing |
Revista: | Microorganisms |
Citação: | Rodriguez, R.; Santos, Carla; Simões, Marta Filipa; Soares, Célia; Santos, Cledir; Lima, Nelson, Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains. Microorganisms, 7(9), 291, 2019 |
Resumo(s): | This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/61259 |
DOI: | 10.3390/microorganisms7090291 |
ISSN: | 2076-2607 |
e-ISSN: | 2076-2607 |
Versão da editora: | http://www.mdpi.com/journal/microorganisms |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51957_1.pdf | 2,63 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons