Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/61259
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Campo DC | Valor | Idioma |
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dc.contributor.author | Rodriguez, R. | por |
dc.contributor.author | Santos, Carla | por |
dc.contributor.author | Simões, Marta Filipa | por |
dc.contributor.author | Soares, Célia | por |
dc.contributor.author | Santos, Cledir | por |
dc.contributor.author | Lima, Nelson | por |
dc.date.accessioned | 2019-09-02T09:18:27Z | - |
dc.date.available | 2019-09-02T09:18:27Z | - |
dc.date.issued | 2019 | - |
dc.identifier.citation | Rodriguez, R.; Santos, Carla; Simões, Marta Filipa; Soares, Célia; Santos, Cledir; Lima, Nelson, Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains. Microorganisms, 7(9), 291, 2019 | por |
dc.identifier.issn | 2076-2607 | por |
dc.identifier.uri | https://hdl.handle.net/1822/61259 | - |
dc.description.abstract | This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent. | por |
dc.description.sponsorship | This research was funded by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020—ProgramaOperacionalRegionaldoNorte. Cl.S.(CledirSantos) thanks the Universidad de La Frontera (Temuco, Chile) for the partial funding from the Project DIUFRO PIA19-0001. R.R. thanks to CONICYT/Chile for the MSc grant Folio N◦ 7317076, Application N◦ 73170764. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI Publishing | por |
dc.relation | UID/BIO/04469/2019 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | freeze-drying | por |
dc.subject | accelerated ageing | por |
dc.subject | mass spectrometry | por |
dc.subject | DNA fingerprinting | por |
dc.subject | biotechnological potential | por |
dc.title | Polyphasic, including MALDI-TOF MS, evaluation of freeze-drying long-term preservation on Aspergillus (section Nigri) strains | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/microorganisms | por |
dc.comments | CEB51957 | por |
oaire.citationIssue | 9 | por |
oaire.citationVolume | 7 | por |
dc.date.updated | 2019-09-02T09:04:36Z | - |
dc.identifier.eissn | 2076-2607 | por |
dc.identifier.doi | 10.3390/microorganisms7090291 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Microorganisms | por |
oaire.version | VoR | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51957_1.pdf | 2,63 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons