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https://hdl.handle.net/1822/5695
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Campo DC | Valor | Idioma |
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dc.contributor.author | Simões, M. | - |
dc.contributor.author | Pereira, Maria Olívia | - |
dc.contributor.author | Vieira, M. J. | - |
dc.date.accessioned | 2006-10-20T16:28:40Z | - |
dc.date.available | 2006-10-20T16:28:40Z | - |
dc.date.issued | 2006 | - |
dc.identifier.citation | LOOSDRECHT, M. C. M. van, ed. - INTERNATIONAL CONFERENCE BIOFILM SYSTEMS, 6, Amsterdam, 2006 – “International Conference Biofilm Systems : Proceedings”. [CD-ROM]. [S.l. : s. n., 2006]. | eng |
dc.identifier.uri | https://hdl.handle.net/1822/5695 | - |
dc.description.abstract | This study investigates the phenotype of turbulent (Re = 5200) and laminar (Re = 2000) flow-generated P. fluorescens biofilms. Three P. fluorescens strains, the type strain– ATCC 13525 and two strains isolated from an industrial processing plant - D3-348 and D3-350, were used throughout this study. The isolated strains were used to form single and binary biofilms. The biofilm physiology (metabolic activity, cellular density, mass, extracellular polymeric substances, structural characteristics and outer membrane proteins-OMP expression) was compared. The results indicate that, for every situation, turbulent flow-generated biofilms were more active (P < 0.05), had more mass per cm2 (P < 0.05), a higher cellular density (P < 0.05), distinct morphology, similar matrix proteins (P > 0.1) and identical (isolated strains - single and binary biofilms) and higher (type strain) matrix polysaccharides contents (P < 0.05) than laminar flow-generated biofilms. Flow-generated biofilms formed by the type strain revealed a considerable higher cellular density and amount of matrix polysaccharides than single and binary biofilms formed by the isolated strains (P < 0.05). Similar OMP expression was detected for the several single strains and for the binary situation, not dependent on the hydrodynamic conditions. Binary biofilms revealed an equal coexistence of the isolated strains with apparent neutral interactions. In summary, the biofilms formed by the type strain represent, apparently, the worst situation in a context of control. The results obtained clearly illustrate the importance of considering strain variation and hydrodynamics in biofilm development and complements previous studies which have focused on physical aspects of structural and density differences. | eng |
dc.description.sponsorship | IBQF, and the Portuguese Foundation for Science and Technology (FCT) Department of Food Science, Cornell University, Ithaca, N.Y. | eng |
dc.language.iso | eng | eng |
dc.relation.ispartofseries | 2006 | eng |
dc.rights | openAccess | eng |
dc.subject | Binary biofilms | eng |
dc.subject | Biofilm structure and activity | eng |
dc.subject | Hydrodynamic conditions | eng |
dc.subject | Phenotypic characterization | eng |
dc.subject | Strain variation | eng |
dc.subject | Turbulent and laminar flows | eng |
dc.title | The role of hydrodynamic stress on the phenotypic characteristics of single and binary biofilms of Pseudomonas fluorescens | eng |
dc.type | conferencePaper | eng |
dc.peerreviewed | yes | eng |
Aparece nas coleções: | CEB - Artigos em Livros de Atas / Papers in Proceedings |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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079[2].pdf | 2,81 MB | Adobe PDF | Ver/Abrir |