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https://hdl.handle.net/1822/56428
Título: | Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for diagnosis of bacterial vaginosis |
Autor(es): | Machado, António Cerca, Nuno |
Palavras-chave: | Fluorescence in situ hybridization (FISH) Lactobacillus spp. Gardnerella vaginalis Vaginal samples Culture cell line Bacterial vaginosis |
Data: | 2017 |
Editora: | Springer |
Revista: | Methods in Molecular Biology |
Citação: | Machado, António; Cerca, Nuno, Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis. In Kimberly A. Bishop-Lilly (Ed.), Diagnostic Bacteriology: Methods and Protocols, Vol. Methods in Molecular Biology 1616, Springer New York, 2017. ISBN: 978-1-4939-7037-7, 209-219 |
Resumo(s): | Fluorescence in situ hybridization (FISH) is a molecular method used to identify and quantify microorganisms in a wide range of samples. This technique combines the simplicity of microscopic observation and the specificity of DNA/rRNA hybridization, allowing detection of selected bacterial species and morphologic visualization. Here, we describe a quantitative molecular diagnosis of bacterial vaginosis, based on the classical Nugent score. Our probes are able to differentiate Lactobacillus spp. and Gardnerella vaginalis from the other undefined bacterial species considered in the Nugent score. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/56428 |
ISBN: | 978-1-4939-7037-7 |
DOI: | 10.1007/978-1-4939-7037-7_13 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/book/10.1007/978-1-4939-7037-7 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
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