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https://hdl.handle.net/1822/56428
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Campo DC | Valor | Idioma |
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dc.contributor.author | Machado, António | por |
dc.contributor.author | Cerca, Nuno | por |
dc.date.accessioned | 2018-10-20T19:06:17Z | - |
dc.date.issued | 2017 | - |
dc.identifier.citation | Machado, António; Cerca, Nuno, Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for Diagnosis of Bacterial Vaginosis. In Kimberly A. Bishop-Lilly (Ed.), Diagnostic Bacteriology: Methods and Protocols, Vol. Methods in Molecular Biology 1616, Springer New York, 2017. ISBN: 978-1-4939-7037-7, 209-219 | por |
dc.identifier.isbn | 978-1-4939-7037-7 | por |
dc.identifier.issn | 1064-3745 | por |
dc.identifier.uri | https://hdl.handle.net/1822/56428 | - |
dc.description.abstract | Fluorescence in situ hybridization (FISH) is a molecular method used to identify and quantify microorganisms in a wide range of samples. This technique combines the simplicity of microscopic observation and the specificity of DNA/rRNA hybridization, allowing detection of selected bacterial species and morphologic visualization. Here, we describe a quantitative molecular diagnosis of bacterial vaginosis, based on the classical Nugent score. Our probes are able to differentiate Lactobacillus spp. and Gardnerella vaginalis from the other undefined bacterial species considered in the Nugent score. | por |
dc.description.sponsorship | Research on BV biofilms in NC laboratory is supported by funding from the Fundação para a Ciência e a Tecnologia (FCT) strategic project of unit UID/BIO/04469/2013. | por |
dc.language.iso | eng | por |
dc.publisher | Springer | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.rights | restrictedAccess | por |
dc.subject | Fluorescence in situ hybridization (FISH) | por |
dc.subject | Lactobacillus spp. | por |
dc.subject | Gardnerella vaginalis | por |
dc.subject | Vaginal samples | por |
dc.subject | Culture cell line | por |
dc.subject | Bacterial vaginosis | por |
dc.title | Multiplex Peptide Nucleic Acid Fluorescence In Situ Hybridization (PNA-FISH) for diagnosis of bacterial vaginosis | por |
dc.type | bookPart | por |
dc.peerreviewed | yes | - |
dc.relation.publisherversion | https://link.springer.com/book/10.1007/978-1-4939-7037-7 | por |
dc.comments | CEB46809 | por |
oaire.citationStartPage | 209 | por |
oaire.citationEndPage | 219 | por |
oaire.citationConferencePlace | New York, NY | - |
oaire.citationVolume | Methods in Molecular Biology 1616 | - |
dc.date.updated | 2018-10-20T18:55:25Z | - |
dc.identifier.doi | 10.1007/978-1-4939-7037-7_13 | por |
dc.identifier.pmid | 28600771 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
sdum.journal | Methods in Molecular Biology | por |
sdum.bookTitle | Diagnostic Bacteriology: Methods and Protocols | por |
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