Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/48482
Título: | Detection of ALK fusion transcripts in FFPE lung cancer samples by NanoString technology |
Autor(es): | Evangelista, Adriane F. Zanon, Maicon F. Carloni, Adriana C. Paula, Flávia Escremim de Morini, Mariana Andozia Ferreira-Neto, Maressa Soares, Iberê Cauduro Miziara, Jose Elias Marchi, Pedro de Scapulatempo-Neto, Cristovam Reis, R. M. |
Palavras-chave: | ALK ALK fusions NanoString FFPE |
Data: | 2017 |
Editora: | BioMed Central (BMC) |
Revista: | BMC Pulmonary Medicine |
Resumo(s): | Background: ALK-rearranged lung cancers exhibit specific pathologic and clinical features and are responsive to anti-ALK therapies. Therefore, the detection of ALK-rearrangement is fundamental for personalized lung cancer therapy. Recently, new molecular techniques, such as NanoString nCounter, have been developed to detect ALK fusions with more accuracy and sensitivity. Methods: In the present study, we intended to validate a NanoString nCounter ALK-fusion panel in routine biopsies of FFPE lung cancer patients. A total of 43 samples were analyzed, 13 ALK-positive and 30 ALK-negative, as previously detected by FISH and/or immunohistochemistry. Results: The NanoString panel detected the presence of the EML4-ALK, KIF5B-ALK and TFG-ALK fusion variants. We observed that all the 13 ALK-positive cases exhibited genetic aberrations by the NanoString methodology. Namely, six cases (46.15%) presented EML-ALK variant 1, two (15.38%) presented EML-ALK variant 2, two (15.38%) presented EML-ALK variant 3a, and three (23.07%) exhibited no variant but presented unbalanced expression between 5'/3' exons, similar to other positive samples. Importantly, for all these analyses, the initial input of RNA was 100 ng, and some cases displayed poor RNA quality measurements. Conclusions: In this study, we reported the great utility of NanoString technology in the assessment of ALK fusions in routine lung biopsies of FFPE specimens. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/48482 |
DOI: | 10.1186/s12890-017-0428-0 |
ISSN: | 1471-2466 |
Versão da editora: | https://bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-017-0428-0 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |