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https://hdl.handle.net/1822/48482
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Campo DC | Valor | Idioma |
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dc.contributor.author | Evangelista, Adriane F. | por |
dc.contributor.author | Zanon, Maicon F. | por |
dc.contributor.author | Carloni, Adriana C. | por |
dc.contributor.author | Paula, Flávia Escremim de | por |
dc.contributor.author | Morini, Mariana Andozia | por |
dc.contributor.author | Ferreira-Neto, Maressa | por |
dc.contributor.author | Soares, Iberê Cauduro | por |
dc.contributor.author | Miziara, Jose Elias | por |
dc.contributor.author | Marchi, Pedro de | por |
dc.contributor.author | Scapulatempo-Neto, Cristovam | por |
dc.contributor.author | Reis, R. M. | por |
dc.date.accessioned | 2017-12-21T10:28:21Z | - |
dc.date.available | 2017-12-21T10:28:21Z | - |
dc.date.issued | 2017 | - |
dc.identifier.issn | 1471-2466 | - |
dc.identifier.uri | https://hdl.handle.net/1822/48482 | - |
dc.description.abstract | Background: ALK-rearranged lung cancers exhibit specific pathologic and clinical features and are responsive to anti-ALK therapies. Therefore, the detection of ALK-rearrangement is fundamental for personalized lung cancer therapy. Recently, new molecular techniques, such as NanoString nCounter, have been developed to detect ALK fusions with more accuracy and sensitivity. Methods: In the present study, we intended to validate a NanoString nCounter ALK-fusion panel in routine biopsies of FFPE lung cancer patients. A total of 43 samples were analyzed, 13 ALK-positive and 30 ALK-negative, as previously detected by FISH and/or immunohistochemistry. Results: The NanoString panel detected the presence of the EML4-ALK, KIF5B-ALK and TFG-ALK fusion variants. We observed that all the 13 ALK-positive cases exhibited genetic aberrations by the NanoString methodology. Namely, six cases (46.15%) presented EML-ALK variant 1, two (15.38%) presented EML-ALK variant 2, two (15.38%) presented EML-ALK variant 3a, and three (23.07%) exhibited no variant but presented unbalanced expression between 5'/3' exons, similar to other positive samples. Importantly, for all these analyses, the initial input of RNA was 100 ng, and some cases displayed poor RNA quality measurements. Conclusions: In this study, we reported the great utility of NanoString technology in the assessment of ALK fusions in routine lung biopsies of FFPE specimens. | por |
dc.description.sponsorship | This study was partially funded by FINEP (MCTI/FINEP/MS/SCTIE/DECIT), Brazil. BIOPLAT (1302/13). | por |
dc.language.iso | eng | por |
dc.publisher | BioMed Central (BMC) | por |
dc.relation | MCTI/FINEP/MS/SCTIE/DECIT | por |
dc.rights | openAccess | por |
dc.subject | ALK | por |
dc.subject | ALK fusions | por |
dc.subject | NanoString | por |
dc.subject | FFPE | por |
dc.title | Detection of ALK fusion transcripts in FFPE lung cancer samples by NanoString technology | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://bmcpulmmed.biomedcentral.com/articles/10.1186/s12890-017-0428-0 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 17 | por |
dc.date.updated | 2017-12-18T12:05:16Z | - |
dc.identifier.doi | 10.1186/s12890-017-0428-0 | por |
dc.identifier.pmid | 28549458 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | BMC Pulmonary Medicine | por |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |