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https://hdl.handle.net/1822/41411
Título: | Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity |
Autor(es): | Oliveira, Hugo Alexandre Mendes Boas, Diana Patrícia Andrade Vilas Mesnage, Stéphane Kluskens, Leon Lavigne, Rob Sillankorva, Sanna Secundo, Francesco Azeredo, Joana |
Palavras-chave: | Acinetobacter baumannii Phage endolysins Mass spectrometry Circular dichroism Antibacterial activity |
Data: | Fev-2016 |
Editora: | Frontiers Media |
Revista: | Frontiers in Microbiology |
Citação: | Oliveira, Hugo; Vilas Boas, Diana; Mesnage, Stéphane; Kluskens, Leon; Lavigne, R.; Sillankorva, Sanna; Secundo, Francesco; Azeredo, Joana, Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity. Frontiers in Microbiology, 7(208), 1-9, 2016 |
Resumo(s): | The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly against multidrug resistant strains of Acinetobacter baumannii. We have cloned, heterologously expressed and characterized a novel endolysin (ABgp46) from Acinetobacter phage vb_AbaP_CEB1 and tested its antibacterial activity against several multidrug-resistant A. baumannii strains. LC-MS revealed that ABgp46 is an N-acetylmuramidase, that is also active over a broad pH range (4.0-10.0) and temperatures up to 50°C. Interestingly, ABgp46 has intrinsic and specific anti-A. baumannii activity, reducing multidrug resistant strains by up to 2 logs within 2 hours. By combining ABgp46 with several organic acids that act as outer membrane permeabilizing agents, it is possible to increase and broaden antibacterial activity to include other Gram-negative bacterial pathogens. In the presence of citric and malic acid, ABgp46 reduces A. baumannii below the detection limit (> 5 log) and more than 4 logs P. aeruginosa and Salmonella Typhimurium strains. Overall, this globular endolysin exhibits a broad and high activity against Gram-negative pathogens, that can be enhanced in presence of citric and malic acid, and be used in human and veterinary medicine. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/41411 |
DOI: | 10.3389/fmicb.2016.00208 |
ISSN: | 1664-302X |
e-ISSN: | 1664-302X |
Versão da editora: | http://journal.frontiersin.org/article/10.3389/fmicb.2016.00208/full |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_37838_1.pdf | 429,04 kB | Adobe PDF | Ver/Abrir |