Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/41411
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, Hugo Alexandre Mendes | por |
dc.contributor.author | Boas, Diana Patrícia Andrade Vilas | por |
dc.contributor.author | Mesnage, Stéphane | por |
dc.contributor.author | Kluskens, Leon | por |
dc.contributor.author | Lavigne, Rob | por |
dc.contributor.author | Sillankorva, Sanna | por |
dc.contributor.author | Secundo, Francesco | por |
dc.contributor.author | Azeredo, Joana | por |
dc.date.accessioned | 2016-05-03T14:54:54Z | - |
dc.date.available | 2016-05-03T14:54:54Z | - |
dc.date.issued | 2016-02 | - |
dc.date.submitted | 2016-01 | - |
dc.identifier.citation | Oliveira, Hugo; Vilas Boas, Diana; Mesnage, Stéphane; Kluskens, Leon; Lavigne, R.; Sillankorva, Sanna; Secundo, Francesco; Azeredo, Joana, Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity. Frontiers in Microbiology, 7(208), 1-9, 2016 | por |
dc.identifier.issn | 1664-302X | por |
dc.identifier.uri | https://hdl.handle.net/1822/41411 | - |
dc.description.abstract | The present study demonstrates the antibacterial potential of a phage endolysin against Gram-negative pathogens, particularly against multidrug resistant strains of Acinetobacter baumannii. We have cloned, heterologously expressed and characterized a novel endolysin (ABgp46) from Acinetobacter phage vb_AbaP_CEB1 and tested its antibacterial activity against several multidrug-resistant A. baumannii strains. LC-MS revealed that ABgp46 is an N-acetylmuramidase, that is also active over a broad pH range (4.0-10.0) and temperatures up to 50°C. Interestingly, ABgp46 has intrinsic and specific anti-A. baumannii activity, reducing multidrug resistant strains by up to 2 logs within 2 hours. By combining ABgp46 with several organic acids that act as outer membrane permeabilizing agents, it is possible to increase and broaden antibacterial activity to include other Gram-negative bacterial pathogens. In the presence of citric and malic acid, ABgp46 reduces A. baumannii below the detection limit (> 5 log) and more than 4 logs P. aeruginosa and Salmonella Typhimurium strains. Overall, this globular endolysin exhibits a broad and high activity against Gram-negative pathogens, that can be enhanced in presence of citric and malic acid, and be used in human and veterinary medicine. | por |
dc.description.sponsorship | Project “BioHealth – Biotechnology and Bioengineering approaches to improve health quality,” Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON.2 – O Novo Norte), QREN, FEDER. The authors also acknowledge the project “Consolidating Research Expertise and Resources on Cellular and Molecular Biotechnology at CEB/IBB,” Ref. FCOMP-01-0124-FEDER-027462 and the bilateral project CNR/FCT. Mass spectrometry analyses were carried out at the Faculty of Science Mass Spectrometry Centre, University of Sheffield | por |
dc.language.iso | eng | por |
dc.publisher | Frontiers Media | por |
dc.rights | openAccess | por |
dc.subject | Acinetobacter baumannii | por |
dc.subject | Phage endolysins | por |
dc.subject | Mass spectrometry | por |
dc.subject | Circular dichroism | por |
dc.subject | Antibacterial activity | por |
dc.title | Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://journal.frontiersin.org/article/10.3389/fmicb.2016.00208/full | por |
dc.comments | CEB37838 | por |
sdum.publicationstatus | info:eu-repo/semantics/publishedVersion | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 9 | por |
oaire.citationIssue | 208 | por |
oaire.citationTitle | Frontiers in Microbiology | por |
oaire.citationVolume | 7 | por |
dc.date.updated | 2016-04-05T18:15:12Z | - |
dc.identifier.eissn | 1664-302X | - |
dc.identifier.doi | 10.3389/fmicb.2016.00208 | por |
dc.subject.fos | Ciências Agrárias::Biotecnologia Agrária e Alimentar | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Frontiers in Microbiology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_37838_1.pdf | 429,04 kB | Adobe PDF | Ver/Abrir |