Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/3375
Título: | Validation of respirometry as a short-term method to assess the efficacy of biocides |
Autor(es): | Simões, M. Pereira, Maria Olívia Vieira, M. J. |
Palavras-chave: | Respiratory activity Live/Dead BacLightTM Culturability Viability Disinfection Biocide Live/Dead BacLight™ |
Data: | 2005 |
Editora: | Taylor and Francis |
Revista: | Biofouling |
Citação: | "Biofouling : The journal of bioadhesion and biofilm research". ISSN 0892-7014. 21:1 (2005) 9-17. |
Resumo(s): | This study shows that a short-term respirometric measurement based on the rate of oxygen uptake needed to oxidize glucose is a reliable and fast method to assess biocide efficacy against P. fluorescens cells. Respiratory activity using oxygen consumption rate, the determination of viable and nonviable cells using Live/Dead® BacLightTM kit and colony formation units (CFU), were compared as indicators of the biocidal efficacy of ortho-phthalaldehyde (OPA). The results showed tha determining the effect of OPA against P. fluorescens using the different methods leads to different conclusions. The minimum bactericidal concentration (MBC) was 80 mg l-¹, 100 mg l-¹ and 65 mg l-¹ respectively, using respiratory activity, viability using BacLight counts and culturability. The plate count method was shown to underestimate the biocidal action of OPA, whilst data from respirometry and viability using Live/Dead BacLight kit correlated strongly and were not statistically different when yellow cells were considered nonviable. Respirometry therefore represents an expeditious, non-destructive and accurate method to determine the antimicrobial action of biocides against aerobic heterotrophic bacteria. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/3375 |
DOI: | 10.1080/08927010500066982 |
ISSN: | 0892-7014 |
Versão da editora: | http://journalsonline.tandf.co.uk/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Biofouling2005-Simoes[2].pdf | 722,06 kB | Adobe PDF | Ver/Abrir |