Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/3375
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Simões, M. | - |
dc.contributor.author | Pereira, Maria Olívia | - |
dc.contributor.author | Vieira, M. J. | - |
dc.date.accessioned | 2005-10-31T14:03:23Z | - |
dc.date.available | 2005-10-31T14:03:23Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | "Biofouling : The journal of bioadhesion and biofilm research". ISSN 0892-7014. 21:1 (2005) 9-17. | eng |
dc.identifier.issn | 0892-7014 | eng |
dc.identifier.uri | https://hdl.handle.net/1822/3375 | - |
dc.description.abstract | This study shows that a short-term respirometric measurement based on the rate of oxygen uptake needed to oxidize glucose is a reliable and fast method to assess biocide efficacy against P. fluorescens cells. Respiratory activity using oxygen consumption rate, the determination of viable and nonviable cells using Live/Dead® BacLightTM kit and colony formation units (CFU), were compared as indicators of the biocidal efficacy of ortho-phthalaldehyde (OPA). The results showed tha determining the effect of OPA against P. fluorescens using the different methods leads to different conclusions. The minimum bactericidal concentration (MBC) was 80 mg l-¹, 100 mg l-¹ and 65 mg l-¹ respectively, using respiratory activity, viability using BacLight counts and culturability. The plate count method was shown to underestimate the biocidal action of OPA, whilst data from respirometry and viability using Live/Dead BacLight kit correlated strongly and were not statistically different when yellow cells were considered nonviable. Respirometry therefore represents an expeditious, non-destructive and accurate method to determine the antimicrobial action of biocides against aerobic heterotrophic bacteria. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.language.iso | eng | eng |
dc.publisher | Taylor and Francis | eng |
dc.rights | openAccess | eng |
dc.subject | Respiratory activity | eng |
dc.subject | Live/Dead BacLightTM | eng |
dc.subject | Culturability | eng |
dc.subject | Viability | eng |
dc.subject | Disinfection | eng |
dc.subject | Biocide | eng |
dc.subject | Live/Dead BacLight™ | por |
dc.title | Validation of respirometry as a short-term method to assess the efficacy of biocides | eng |
dc.type | article | por |
dc.peerreviewed | yes | eng |
dc.relation.publisherversion | http://journalsonline.tandf.co.uk/ | eng |
sdum.number | 1 | eng |
sdum.pagination | 9-17 | eng |
sdum.publicationstatus | published | eng |
sdum.volume | 21 | eng |
oaire.citationStartPage | 9 | por |
oaire.citationEndPage | 17 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 21 | por |
dc.identifier.doi | 10.1080/08927010500066982 | por |
dc.identifier.pmid | 16019387 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Biofouling | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Biofouling2005-Simoes[2].pdf | 722,06 kB | Adobe PDF | Ver/Abrir |