Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/32358
Título: | Molecularly engineered self-assembling membranes for cell-mediated degradation |
Autor(es): | Ferreira , Daniela S. Lin, Yi-An Cui, Honggang Hubbell, Jeffrey A. Reis, R. L. Azevedo, Helena S. |
Palavras-chave: | Degradation Enzyme-responsive materials Hyaluronan Matrix metalloproteinase-1 Peptide amphiphiles Self-assembling membranes |
Data: | 2015 |
Editora: | Wiley-VCH Verlag |
Revista: | Advanced Healthcare Materials |
Citação: | Ferreira, D. S., Lin, Y. A., Cui, H., Hubbell, J. A., Reis, R. L., & Azevedo, H. S. (2015). Molecularly engineered self-assembling membranes for cell-mediated degradation. Advanced Healthcare Materials, 4(4), 602-612. doi: 10.1002/adhm.201400586 |
Resumo(s): | The use of peptide engineering to develop self-assembling membranes that are responsive to cellular enzyme activities is reported. The membranes are obtained by combining hyaluronan (HA) and a rationally designed peptide amphiphile (PA) containing a proteolytic domain (GPQGIWGQ octapeptide) sensitive to matrix metalloproteinase-1 (MMP-1). Insertion of an octapeptide in a typical PA structure does not disturb its self-assembly into fibrillar nanostructures neither the ability to form membranes with HA. In vitro enzymatic degradation with hyaluronidase and MMP-1 shows that membranes containing the MMP-1 substrate exhibit enhanced enzymatic degradation, compared with control membranes (absence of MMP-1 cleavable peptide or containing a MMP-1 insensitive sequence), being completely degraded after 7 days. Cell viability and proliferation is minimally affected by the enzymatically cleavable functionality of the membrane, but the presence of MMP-1 cleavable sequence does stimulate the secretion of MMP-1 by fibroblasts and interfere with matrix deposition, particularly the deposition of collagen. By showing cell-responsiveness to biochemical signals presented on self-assembling membranes, this study highlights the ability of modulating certain cellular activities through matrix engineering. This concept can be further explored to understand the cellular remodeling process and as a strategy to develop artificial matrices with more biomimetic degradation for tissue engineering applications. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/32358 |
DOI: | 10.1002/adhm.201400586 |
ISSN: | 2192-2659 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18278-MolecularlyEngineeredSAMembranes_Cell-mediatedDegradation_DSFerreira_AdvHealthcareMater2014.pdf Acesso restrito! | 4,36 MB | Adobe PDF | Ver/Abrir |