Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/32358
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Ferreira , Daniela S. | por |
dc.contributor.author | Lin, Yi-An | por |
dc.contributor.author | Cui, Honggang | por |
dc.contributor.author | Hubbell, Jeffrey A. | por |
dc.contributor.author | Reis, R. L. | por |
dc.contributor.author | Azevedo, Helena S. | por |
dc.date.accessioned | 2015-01-06T10:06:22Z | - |
dc.date.available | 2015-01-06T10:06:22Z | - |
dc.date.issued | 2015 | - |
dc.date.submitted | 2014-12 | - |
dc.identifier.citation | Ferreira, D. S., Lin, Y. A., Cui, H., Hubbell, J. A., Reis, R. L., & Azevedo, H. S. (2015). Molecularly engineered self-assembling membranes for cell-mediated degradation. Advanced Healthcare Materials, 4(4), 602-612. doi: 10.1002/adhm.201400586 | por |
dc.identifier.issn | 2192-2659 | por |
dc.identifier.uri | https://hdl.handle.net/1822/32358 | - |
dc.description.abstract | The use of peptide engineering to develop self-assembling membranes that are responsive to cellular enzyme activities is reported. The membranes are obtained by combining hyaluronan (HA) and a rationally designed peptide amphiphile (PA) containing a proteolytic domain (GPQGIWGQ octapeptide) sensitive to matrix metalloproteinase-1 (MMP-1). Insertion of an octapeptide in a typical PA structure does not disturb its self-assembly into fibrillar nanostructures neither the ability to form membranes with HA. In vitro enzymatic degradation with hyaluronidase and MMP-1 shows that membranes containing the MMP-1 substrate exhibit enhanced enzymatic degradation, compared with control membranes (absence of MMP-1 cleavable peptide or containing a MMP-1 insensitive sequence), being completely degraded after 7 days. Cell viability and proliferation is minimally affected by the enzymatically cleavable functionality of the membrane, but the presence of MMP-1 cleavable sequence does stimulate the secretion of MMP-1 by fibroblasts and interfere with matrix deposition, particularly the deposition of collagen. By showing cell-responsiveness to biochemical signals presented on self-assembling membranes, this study highlights the ability of modulating certain cellular activities through matrix engineering. This concept can be further explored to understand the cellular remodeling process and as a strategy to develop artificial matrices with more biomimetic degradation for tissue engineering applications. | por |
dc.description.sponsorship | This work was funded by the European Regional Development Fund (ERDF) through the Operational Competitiveness Programme "COMPETE" (FCOMP-01-0124-FEDER-014758) and national funds through the Portuguese Foundation for Science and Technology (FCT) under the project PTDC/EBB-BIO/114523/2009. The authors also thank a start-up grant provided by the School of Engineering and Materials Science at QMUL. D.S.F. gratefully acknowledges FCT for the PhD scholarship (SFRH/BD/44977/2008). | por |
dc.language.iso | eng | por |
dc.publisher | Wiley-VCH Verlag | por |
dc.rights | restrictedAccess | por |
dc.subject | Degradation | por |
dc.subject | Enzyme-responsive materials | por |
dc.subject | Hyaluronan | por |
dc.subject | Matrix metalloproteinase-1 | por |
dc.subject | Peptide amphiphiles | por |
dc.subject | Self-assembling membranes | por |
dc.title | Molecularly engineered self-assembling membranes for cell-mediated degradation | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | http://www.3bs.uminho.pt/node/18278 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 602 | por |
oaire.citationEndPage | 612 | por |
oaire.citationIssue | 4 | por |
oaire.citationTitle | Advanced Healthcare Materials | por |
oaire.citationVolume | 4 | por |
dc.date.updated | 2014-12-22T14:24:27Z | - |
dc.identifier.doi | 10.1002/adhm.201400586 | por |
dc.identifier.pmid | 25413155 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Advanced Healthcare Materials | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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18278-MolecularlyEngineeredSAMembranes_Cell-mediatedDegradation_DSFerreira_AdvHealthcareMater2014.pdf Acesso restrito! | 4,36 MB | Adobe PDF | Ver/Abrir |