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https://hdl.handle.net/1822/31597
Título: | A thermostable salmonella phage endolysin, Lys68, with broad bactericidal properties against gram-negative pathogens in presence of weak acids |
Autor(es): | Oliveira, Hugo Thiagarajan, V. Walmagh, Maarten Sillankorva, Sanna Lavigne, Rob Petersen, T. Neves Kluskens, Leon Azeredo, Joana |
Data: | 2014 |
Editora: | Public Library of Science |
Revista: | PLoS ONE |
Citação: | Oliveira, Hugo; Thiagarajan, V.; Walmagh, M.; Sillankorva, Sanna; Lavigne, R.; Neves-Petersen, M. T.; Kluskens, Leon; Azeredo, Joana, A thermostable salmonella phage endolysin, Lys68, with broad bactericidal properties against gram-negative pathogens in presence of weak acids. PLoS One, 9(10), e108376, 2014 |
Resumo(s): | Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This paper characterizes a Salmonella phage endolysin (Lys68) and demonstrates its potential antimicrobial effectiveness when combined with organic acids towards Gram-negative pathogens. Biochemical characterization reveals that Lys68 is more active at pH 7.0, maintaining 76.7% of its activity when stored at 4°C for two months. Thermostability tests showed that Lys68 is only completely inactivated upon exposure to 100°C for 30 min, and circular dichroism analysis demonstrated the ability to refold into its original conformation upon thermal denaturation. It was shown that Lys68 is able to lyse a wide panel of Gram-negative bacteria (13 different species) in combination with the outer membrane permeabilizers EDTA, citric and malic acid. While the EDTA/Lys68 combination only inactivated Pseudomonas strains, the use of citric or malic acid broadened Lys68 antibacterial effect to other Gram-negative pathogens (lytic activity against 9 and 11 species, respectively). Particularly against Salmonella Typhimurium LT2, the combinatory effect of malic or citric acid with Lys68 led to approximately 3 to 5 log reductions in bacterial load/CFUs after 2 hours, respectively, and was also able to reduce stationary-phase cells and bacterial biofilms by approximately 1 log. The broad killing capacity of malic/citric acid-Lys68 is explained by the destabilization and major disruptions of the cell outer membrane integrity due to the acidity caused by the organic acids and a relatively high muralytic activity of Lys68 at low pH. Lys68 demonstrates good (thermo)stability properties that combined with different outer membrane permeabilizers, could become useful to combat Gram-negative pathogens in agricultural, food and medical industry. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31597 |
DOI: | 10.1371/journal.pone.0108376 |
ISSN: | 1932-6203 |
e-ISSN: | 1932-6203 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18173_1.pdf | 685,17 kB | Adobe PDF | Ver/Abrir |