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https://hdl.handle.net/1822/31597
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Campo DC | Valor | Idioma |
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dc.contributor.author | Oliveira, Hugo | por |
dc.contributor.author | Thiagarajan, V. | por |
dc.contributor.author | Walmagh, Maarten | por |
dc.contributor.author | Sillankorva, Sanna | por |
dc.contributor.author | Lavigne, Rob | por |
dc.contributor.author | Petersen, T. Neves | por |
dc.contributor.author | Kluskens, Leon | por |
dc.contributor.author | Azeredo, Joana | por |
dc.date.accessioned | 2014-12-03T17:52:45Z | - |
dc.date.available | 2014-12-03T17:52:45Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Oliveira, Hugo; Thiagarajan, V.; Walmagh, M.; Sillankorva, Sanna; Lavigne, R.; Neves-Petersen, M. T.; Kluskens, Leon; Azeredo, Joana, A thermostable salmonella phage endolysin, Lys68, with broad bactericidal properties against gram-negative pathogens in presence of weak acids. PLoS One, 9(10), e108376, 2014 | por |
dc.identifier.issn | 1932-6203 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31597 | - |
dc.description.abstract | Resistance rates are increasing among several problematic Gram-negative pathogens, a fact that has encouraged the development of new antimicrobial agents. This paper characterizes a Salmonella phage endolysin (Lys68) and demonstrates its potential antimicrobial effectiveness when combined with organic acids towards Gram-negative pathogens. Biochemical characterization reveals that Lys68 is more active at pH 7.0, maintaining 76.7% of its activity when stored at 4°C for two months. Thermostability tests showed that Lys68 is only completely inactivated upon exposure to 100°C for 30 min, and circular dichroism analysis demonstrated the ability to refold into its original conformation upon thermal denaturation. It was shown that Lys68 is able to lyse a wide panel of Gram-negative bacteria (13 different species) in combination with the outer membrane permeabilizers EDTA, citric and malic acid. While the EDTA/Lys68 combination only inactivated Pseudomonas strains, the use of citric or malic acid broadened Lys68 antibacterial effect to other Gram-negative pathogens (lytic activity against 9 and 11 species, respectively). Particularly against Salmonella Typhimurium LT2, the combinatory effect of malic or citric acid with Lys68 led to approximately 3 to 5 log reductions in bacterial load/CFUs after 2 hours, respectively, and was also able to reduce stationary-phase cells and bacterial biofilms by approximately 1 log. The broad killing capacity of malic/citric acid-Lys68 is explained by the destabilization and major disruptions of the cell outer membrane integrity due to the acidity caused by the organic acids and a relatively high muralytic activity of Lys68 at low pH. Lys68 demonstrates good (thermo)stability properties that combined with different outer membrane permeabilizers, could become useful to combat Gram-negative pathogens in agricultural, food and medical industry. | por |
dc.description.sponsorship | This work was supported by the projects FCOMP-01-0124-FEDER-019446, FCOMP-01-0124-FEDER-027462 and PEst-OE/EQB/LA0023/2013 from "Fundacao para a Ciencia e Tecnologia" (FCT), Portugal. The authors thank the Project "BioHealth - Biotechnology and Bioengineering approaches to improve health quality", Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON. 2 - O Novo Norte), QREN, FEDER. Hugo Oliveira acknowledges the FCT grant SFRH/BD/63734/2009. Maarten Walmagh held a PhD scholarship of the IWT Vlaanderen. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | por |
dc.language.iso | eng | por |
dc.publisher | Public Library of Science | por |
dc.rights | openAccess | por |
dc.title | A thermostable salmonella phage endolysin, Lys68, with broad bactericidal properties against gram-negative pathogens in presence of weak acids | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB18173 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | e108376 | por |
oaire.citationIssue | 10 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationTitle | PLoS ONE | por |
oaire.citationVolume | 9 | por |
dc.date.updated | 2014-11-28T17:42:59Z | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.doi | 10.1371/journal.pone.0108376 | por |
dc.identifier.pmid | 25290100 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | PLoS ONE | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18173_1.pdf | 685,17 kB | Adobe PDF | Ver/Abrir |