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https://hdl.handle.net/1822/31469
Título: | Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system |
Autor(es): | Carneiro-da-Cunha, M. N. Souza, K. P. S. Mota, A. Teixeira, J. A. Porto, C. S. Porto, Tatiana Souza Porto, Ana L. F. |
Palavras-chave: | Clavulanic acid ATPS PEG Citrate salts Stability |
Data: | 2014 |
Editora: | Elsevier B.V. |
Revista: | Fluid Phase Equilibria |
Citação: | Carneiro-da-Cunha, M. N.; Souza, K. P. S.; Mota, A.; Teixeira, José A.; Porto, C. S.; Porto, T. S.; Porto, A. L. F., Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system. Fluid Phase Equilibria, 375, 104-109, 2014 |
Resumo(s): | β-Lactamases are enzymes responsible for the hydrolysis of β-lactam antibiotics, being produced by several pathogenic bacteria. Clavulanic acid is a commercially and clinically important β-lactamase inhibitor, its extraction being possible by the application of aqueous two-phase system. In this study, clavulanic acid stability was investigated at different molar mass PEG (400, 1 000 and 20 000 g mol−1) and at different citrate concentrations (5 and 20%) PEG/citrate aqueous-two phase systems (ATPS), under different pH values (4.0–8.0). Clavulanic acid extraction was also evaluated. Low citrate concentration and PEG 20 000 (g mol−1) ATPS, at pH 6.0, were shown to be the systems that presented the highest clavulanic acid stability. Based on this, a factorial design 22 was used to evaluate CA extraction, being PEG (20, 25 and 30%) and citrate (5, 10 and 15%) concentrations the parameters evaluated. Clavulanic acid was extracted into the PEG-rich phase (133.66 mg L−1), the highest values of the partition coefficient and yield being K = 5.92, Y = 103.53%, respectively The ATPS was not only effective on clavulanic acid extraction, but also its degradation was minimal. These results clearly indicate that ATPS can be successfully applied as a first step for the purification of clavulanic acid. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/31469 |
DOI: | 10.1016/j.fluid.2014.04.036 |
ISSN: | 0378-3812 |
e-ISSN: | 0378-3812 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18225_1.pdf | 984,09 kB | Adobe PDF | Ver/Abrir |