Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/31469
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Campo DC | Valor | Idioma |
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dc.contributor.author | Carneiro-da-Cunha, M. N. | por |
dc.contributor.author | Souza, K. P. S. | por |
dc.contributor.author | Mota, A. | por |
dc.contributor.author | Teixeira, J. A. | por |
dc.contributor.author | Porto, C. S. | por |
dc.contributor.author | Porto, Tatiana Souza | por |
dc.contributor.author | Porto, Ana L. F. | por |
dc.date.accessioned | 2014-12-01T15:50:55Z | - |
dc.date.available | 2014-12-01T15:50:55Z | - |
dc.date.issued | 2014 | - |
dc.identifier.citation | Carneiro-da-Cunha, M. N.; Souza, K. P. S.; Mota, A.; Teixeira, José A.; Porto, C. S.; Porto, T. S.; Porto, A. L. F., Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system. Fluid Phase Equilibria, 375, 104-109, 2014 | por |
dc.identifier.issn | 0378-3812 | por |
dc.identifier.uri | https://hdl.handle.net/1822/31469 | - |
dc.description.abstract | β-Lactamases are enzymes responsible for the hydrolysis of β-lactam antibiotics, being produced by several pathogenic bacteria. Clavulanic acid is a commercially and clinically important β-lactamase inhibitor, its extraction being possible by the application of aqueous two-phase system. In this study, clavulanic acid stability was investigated at different molar mass PEG (400, 1 000 and 20 000 g mol−1) and at different citrate concentrations (5 and 20%) PEG/citrate aqueous-two phase systems (ATPS), under different pH values (4.0–8.0). Clavulanic acid extraction was also evaluated. Low citrate concentration and PEG 20 000 (g mol−1) ATPS, at pH 6.0, were shown to be the systems that presented the highest clavulanic acid stability. Based on this, a factorial design 22 was used to evaluate CA extraction, being PEG (20, 25 and 30%) and citrate (5, 10 and 15%) concentrations the parameters evaluated. Clavulanic acid was extracted into the PEG-rich phase (133.66 mg L−1), the highest values of the partition coefficient and yield being K = 5.92, Y = 103.53%, respectively The ATPS was not only effective on clavulanic acid extraction, but also its degradation was minimal. These results clearly indicate that ATPS can be successfully applied as a first step for the purification of clavulanic acid. | por |
dc.description.sponsorship | CNPq -Conselho Nacional de Desenvolvimento Científico e Tecnológico(150763/2013-1) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier B.V. | por |
dc.rights | openAccess | por |
dc.subject | Clavulanic acid | por |
dc.subject | ATPS | por |
dc.subject | PEG | por |
dc.subject | Citrate salts | por |
dc.subject | Stability | por |
dc.title | Stability of clavulanic acid in PEG/citrate and liquid–liquid extraction in aqueous two-phase system | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.comments | CEB18225 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 104 | por |
oaire.citationEndPage | 109 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationTitle | Fluid Phase Equilibria | por |
oaire.citationVolume | 375 | por |
dc.date.updated | 2014-11-28T17:41:47Z | - |
dc.identifier.eissn | 0378-3812 | - |
dc.identifier.doi | 10.1016/j.fluid.2014.04.036 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Fluid Phase Equilibria | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_18225_1.pdf | 984,09 kB | Adobe PDF | Ver/Abrir |