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https://hdl.handle.net/1822/23676
Título: | Rheological and mechanical properties of acellular and cellladen methacrylated gellan gum hydrogels |
Autor(es): | Silva-Correia, Joana Gloria, A. Oliveira, Mariana B. Mano, J. F. Oliveira, Joaquim M. Ambrosio, L. Reis, R. L. |
Palavras-chave: | Hydrogels Injectability Mechanical properties Methacrylated gellan gum Rheology |
Data: | Abr-2013 |
Editora: | John Wiley and Sons |
Revista: | Journal of Biomedical Materials Research: Part A |
Resumo(s): | Tissue engineered hydrogels hold great potential as nucleus pulposus substitutes (NP), as they promote intervertebral disc (IVD) regeneration and re-establish its original function. But, the key to their success in future clinical applications greatly depends on its ability to replicate the native 3D micro-environment and circumvent their limitation in terms of mechanical performance. In the present study, we investigated the rheological/mechanical properties of both ionic- (iGG-MA) and photo-crosslinked methacrylated gellan gum (phGG-MA) hydrogels. Steady shear analysis, injectability and confined compression stress-relaxation tests were carried out. The injectability of the reactive solutions employed for the preparation of iGG-MA and phGG-MA hydrogels was firstly studied, then the zero-strain compressive modulus and permeability of the acellular hydrogels were evaluated.In addition, human intervertebral disc (hIVD) cells encapsulated in both iGG-MA and phGG-MA hydrogels were cultured in vitro, and its mechanical properties also investigated under dynamic mechanical analysis at 37ºC and pH 7.4. After 21 d of culturing, hIVD cells were alive (Calcein AM) and the E’ of ionic-crosslinked hydrogels and photo-crosslinked was higher than that observed for acellular hydrogels. Our study suggests that methacrylated gellan gum hydrogels present promising mechanical and biological performance as hIVD cells were producing extracellular matrix. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/23676 |
DOI: | 10.1002/jbm.a.34650 |
ISSN: | 1552-4965 |
Versão da editora: | http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34650/abstract |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17379-10.1002_jbm.a.34650.pdf | 639,26 kB | Adobe PDF | Ver/Abrir |