Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/23676
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Silva-Correia, Joana | - |
dc.contributor.author | Gloria, A. | - |
dc.contributor.author | Oliveira, Mariana B. | - |
dc.contributor.author | Mano, J. F. | - |
dc.contributor.author | Oliveira, Joaquim M. | - |
dc.contributor.author | Ambrosio, L. | - |
dc.contributor.author | Reis, R. L. | - |
dc.date.accessioned | 2013-04-11T14:52:52Z | - |
dc.date.available | 2013-04-11T14:52:52Z | - |
dc.date.issued | 2013-04 | - |
dc.date.submitted | 2013-02 | - |
dc.identifier.issn | 1552-4965 | por |
dc.identifier.uri | https://hdl.handle.net/1822/23676 | - |
dc.description.abstract | Tissue engineered hydrogels hold great potential as nucleus pulposus substitutes (NP), as they promote intervertebral disc (IVD) regeneration and re-establish its original function. But, the key to their success in future clinical applications greatly depends on its ability to replicate the native 3D micro-environment and circumvent their limitation in terms of mechanical performance. In the present study, we investigated the rheological/mechanical properties of both ionic- (iGG-MA) and photo-crosslinked methacrylated gellan gum (phGG-MA) hydrogels. Steady shear analysis, injectability and confined compression stress-relaxation tests were carried out. The injectability of the reactive solutions employed for the preparation of iGG-MA and phGG-MA hydrogels was firstly studied, then the zero-strain compressive modulus and permeability of the acellular hydrogels were evaluated.In addition, human intervertebral disc (hIVD) cells encapsulated in both iGG-MA and phGG-MA hydrogels were cultured in vitro, and its mechanical properties also investigated under dynamic mechanical analysis at 37ºC and pH 7.4. After 21 d of culturing, hIVD cells were alive (Calcein AM) and the E’ of ionic-crosslinked hydrogels and photo-crosslinked was higher than that observed for acellular hydrogels. Our study suggests that methacrylated gellan gum hydrogels present promising mechanical and biological performance as hIVD cells were producing extracellular matrix. | por |
dc.language.iso | eng | por |
dc.publisher | John Wiley and Sons | por |
dc.rights | openAccess | por |
dc.subject | Hydrogels | por |
dc.subject | Injectability | por |
dc.subject | Mechanical properties | por |
dc.subject | Methacrylated gellan gum | por |
dc.subject | Rheology | por |
dc.title | Rheological and mechanical properties of acellular and cellladen methacrylated gellan gum hydrogels | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.34650/abstract | por |
dc.comments | http://www.3bs.uminho.pt/node/17379 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 3438 | por |
oaire.citationEndPage | 3446 | por |
oaire.citationIssue | 12 | por |
oaire.citationVolume | 101 | por |
dc.date.updated | 2013-04-11T10:09:02Z | - |
dc.identifier.doi | 10.1002/jbm.a.34650 | - |
dc.identifier.pmid | 23568694 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Biomedical Materials Research: Part A | por |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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17379-10.1002_jbm.a.34650.pdf | 639,26 kB | Adobe PDF | Ver/Abrir |