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https://hdl.handle.net/1822/22846
Título: | Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor |
Autor(es): | Maciel, Marília de Holanda Cavalcanti Ottoni, C. A. Santos, C. Lima, Nelson Moreira, Keila Motta, Cristina Souza |
Palavras-chave: | Aspergillus niger Orange peel Immobilization cells Polygalacturonases Fixed bed reactor |
Data: | 2013 |
Editora: | MDPI |
Revista: | Molecules |
Resumo(s): | Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, study a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U·mL-1 and 4.11 U·mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/22846 |
DOI: | 10.3390/molecules18021660 |
ISSN: | 1420-3049 |
Versão da editora: | http://www.mdpi.com/journal/molecules |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2013 Production polygalacturonase Aspergillus section Nigri bed reactor.pdf | artigo | 544,62 kB | Adobe PDF | Ver/Abrir |