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https://hdl.handle.net/1822/22846
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Campo DC | Valor | Idioma |
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dc.contributor.author | Maciel, Marília de Holanda Cavalcanti | - |
dc.contributor.author | Ottoni, C. A. | - |
dc.contributor.author | Santos, C. | - |
dc.contributor.author | Lima, Nelson | - |
dc.contributor.author | Moreira, Keila | - |
dc.contributor.author | Motta, Cristina Souza | - |
dc.date.accessioned | 2013-01-29T11:26:31Z | - |
dc.date.available | 2013-01-29T11:26:31Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1420-3049 | por |
dc.identifier.uri | https://hdl.handle.net/1822/22846 | - |
dc.description.abstract | Polygalacturonases (PG) are pectinolytic enzymes that have technological, functional and biological applications in food processing, fruit ripening and plant-fungus interactions, respectively. In the present, study a microtitre plate methodology was used for rapid screening of 61 isolates of fungi from Aspergillus section Nigri to assess production of endo- and exo-PG. Studies of scale-up were carried out in a fixed bed reactor operated under different parameters using the best producer strain immobilised in orange peels. Four experiments were conducted under the following conditions: the immobilised cells without aeration; immobilised cells with aeration; immobilised cells with aeration and added pectin; and free cells with aeration. The fermentation was performed for 168 h with removal of sample every 24 h. Aspergillus niger strain URM 5162 showed the highest PG production. The results obtained indicated that the maximum endo- and exo-PG activities (1.18 U·mL-1 and 4.11 U·mL-1, respectively) were obtained when the reactor was operating without aeration. The microtitre plate method is a simple way to screen fungal isolates for PG activity detection. The fixed bed reactor with orange peel support and using A. niger URM 5162 is a promising process for PG production at the industrial level. | por |
dc.description.sponsorship | M.H.C. Maciel thanks to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil) and Erasmus Mundus External Cooperation Window Lote 17. | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.rights | openAccess | por |
dc.subject | Aspergillus niger | por |
dc.subject | Orange peel | por |
dc.subject | Immobilization cells | por |
dc.subject | Polygalacturonases | por |
dc.subject | Fixed bed reactor | por |
dc.title | Production of polygalacturonases by aspergillus section Nigri strains in a fixed bed reactor | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/molecules | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 1660 | por |
oaire.citationEndPage | 1671 | por |
oaire.citationIssue | 2 | por |
oaire.citationTitle | Molecules | por |
oaire.citationVolume | 18 | por |
dc.identifier.doi | 10.3390/molecules18021660 | por |
dc.identifier.pmid | 23358324 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Molecules | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2013 Production polygalacturonase Aspergillus section Nigri bed reactor.pdf | artigo | 544,62 kB | Adobe PDF | Ver/Abrir |