Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/18405
Título: | Protective effects of ursolic acid and luteolin against oxidative DNA damage include enhancement of DNA repair in Caco-2 cells |
Autor(es): | Ramos, Alice A. Wilson, Cristina Pereira Collins, Andrew R. |
Palavras-chave: | Ursolic acid Luteolin DNA oxidation Antioxidants DNA repair Comet assay |
Data: | Out-2010 |
Editora: | Elsevier 1 |
Revista: | Mutation Research/fundamental and Molecular Mechanisms of Mutagenesis |
Resumo(s): | Consumption of fruits and vegetables is associated with a reduced risk of developing a wide range of cancers including colon cancer. In this study, we evaluated the effects of two compounds present in fruits and vegetables, ursolic acid, a triterpenoid, and luteolin, a flavonoid, on DNA protection and DNA repair in Caco-2 cells using the comet assay. Ursolic acid and luteolin showed a protective effect against H2O2-induced DNA damage. Repair rate (rejoining of strand breaks) after treatment with H2O2 was increased by pre-treatment of Caco-2 cells for 24 h with ursolic acid or luteolin. To evaluate effects on induction of base oxidation, we exposed cells to the photosensitizer Ro 19-8022 plus visible light to induce 8-oxoguanine. Luteolin protected against this damage in Caco-2 cells after a short period of incubation. We also measured the incision activity of a cell extract from Caco-2 cells treated for 24 h with test compounds, on a DNA substrate containing specific damage (8-oxoGua), to evaluate effects on base excision repair activity. Preincubation for 24 h with ursolic acid enhanced incision activity in Caco-2 cells. In conclusion, we demonstrated for the first time that ursolic acid and luteolin not only protect DNA from oxidative damage but also increase repair activity in Caco-2 cells. These effects of ursolic acid and luteolin may contribute to their anti-carcinogenic effects. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/18405 |
DOI: | 10.1016/j.mrfmmm.2010.07.004 |
ISSN: | 0027-5107 |
Versão da editora: | http://www.sciencedirect.com/science/article/pii/S0027510710002009 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
Ramos_mutation research 2010_post-print.pdf | Manuscript post-print | 425,42 kB | Adobe PDF | Ver/Abrir |