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https://hdl.handle.net/1822/18405
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Campo DC | Valor | Idioma |
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dc.contributor.author | Ramos, Alice A. | - |
dc.contributor.author | Wilson, Cristina Pereira | - |
dc.contributor.author | Collins, Andrew R. | - |
dc.date.accessioned | 2012-04-04T11:21:07Z | - |
dc.date.available | 2012-04-04T11:21:07Z | - |
dc.date.issued | 2010-10 | - |
dc.date.submitted | 2010 | - |
dc.identifier.issn | 0027-5107 | por |
dc.identifier.uri | https://hdl.handle.net/1822/18405 | - |
dc.description.abstract | Consumption of fruits and vegetables is associated with a reduced risk of developing a wide range of cancers including colon cancer. In this study, we evaluated the effects of two compounds present in fruits and vegetables, ursolic acid, a triterpenoid, and luteolin, a flavonoid, on DNA protection and DNA repair in Caco-2 cells using the comet assay. Ursolic acid and luteolin showed a protective effect against H2O2-induced DNA damage. Repair rate (rejoining of strand breaks) after treatment with H2O2 was increased by pre-treatment of Caco-2 cells for 24 h with ursolic acid or luteolin. To evaluate effects on induction of base oxidation, we exposed cells to the photosensitizer Ro 19-8022 plus visible light to induce 8-oxoguanine. Luteolin protected against this damage in Caco-2 cells after a short period of incubation. We also measured the incision activity of a cell extract from Caco-2 cells treated for 24 h with test compounds, on a DNA substrate containing specific damage (8-oxoGua), to evaluate effects on base excision repair activity. Preincubation for 24 h with ursolic acid enhanced incision activity in Caco-2 cells. In conclusion, we demonstrated for the first time that ursolic acid and luteolin not only protect DNA from oxidative damage but also increase repair activity in Caco-2 cells. These effects of ursolic acid and luteolin may contribute to their anti-carcinogenic effects. | por |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.rights | openAccess | por |
dc.subject | Ursolic acid | por |
dc.subject | Luteolin | por |
dc.subject | DNA oxidation | por |
dc.subject | Antioxidants | por |
dc.subject | DNA repair | por |
dc.subject | Comet assay | por |
dc.title | Protective effects of ursolic acid and luteolin against oxidative DNA damage include enhancement of DNA repair in Caco-2 cells | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0027510710002009 | por |
sdum.publicationstatus | published | por |
oaire.citationStartPage | 6 | por |
oaire.citationEndPage | 11 | por |
oaire.citationIssue | 1-2 | por |
oaire.citationTitle | Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis | por |
oaire.citationVolume | 692 | por |
dc.identifier.doi | 10.1016/j.mrfmmm.2010.07.004 | por |
dc.identifier.pmid | 20659486 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Mutation Research/fundamental and Molecular Mechanisms of Mutagenesis | por |
Aparece nas coleções: | DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Ramos_mutation research 2010_post-print.pdf | Manuscript post-print | 425,42 kB | Adobe PDF | Ver/Abrir |