Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1776
Título: | Separation of endo-polygalacturonase using aqueous two-phase partitioning |
Autor(es): | You-Ting Wu Pereira, Martinha Venâncio, Armando Teixeira, J. A. |
Palavras-chave: | Partitioning Aqueous two-phase systems Enzymes Endo-polygalacturonase |
Data: | 2001 |
Editora: | Elsevier 1 |
Revista: | Journal of Chromatography A |
Citação: | “Journal of Chromatography A”. ISSN 0021-9673. 929:1-2 (2001) 23-29. |
Resumo(s): | The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)–polyvinyl alcohol (PVA10 000) and PEG–hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000– PVA10 000 system, endo-PG presented in the fermentation broth (at concentration that is more than 40% of total protein) mainly dominates in the top phase with a partitioning coefficient of 6, while total protein concentrates in the bottom phase. A separation scheme consisting of two consecutive aqueous two-phase extraction steps was proposed: a first extraction in polyethylene glycol (PEG8000)–polyvinyl alcohol system, followed by a second extraction in PEG8000–(NH4)2SO4 system. This allowed the separation of endo-PG from polymer and the recycling of PEG polymer, since endo-PG was very strongly partitioned into the bottom phase of the PEG8000–(NH4)2SO4 system. Laboratory-scale experiments were performed to test the efficiency of this scheme. It was found that enzyme recovery was up to 91% with a total purification factor of about 1.9 and a concentration factor of more than 5. About 90% of the total PEG added into the systems can be recovered, and no reduction was obtained in the purification factor using recycled PEG. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1776 |
DOI: | 10.1016/S0021-9673(01)01161-X |
ISSN: | 0021-9673 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2001-33[1].pdf | 261,5 kB | Adobe PDF | Ver/Abrir |