Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1776
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | You-Ting Wu | - |
dc.contributor.author | Pereira, Martinha | - |
dc.contributor.author | Venâncio, Armando | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.date.accessioned | 2005-05-24T14:20:37Z | - |
dc.date.available | 2005-05-24T14:20:37Z | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | “Journal of Chromatography A”. ISSN 0021-9673. 929:1-2 (2001) 23-29. | eng |
dc.identifier.issn | 0021-9673 | - |
dc.identifier.uri | https://hdl.handle.net/1822/1776 | - |
dc.description.abstract | The partitioning of endo-polygalacturonase (endo-PG) in polyethylene glycol (PEG)–polyvinyl alcohol (PVA10 000) and PEG–hydroxypropyl starch (Reppal PES100) aqueous two-phase systems was studied, and revealed the possibility of using aqueous two-phase extraction to purify and concentrate endo-PG from its clarified fermentation broth. For the PEG8000– PVA10 000 system, endo-PG presented in the fermentation broth (at concentration that is more than 40% of total protein) mainly dominates in the top phase with a partitioning coefficient of 6, while total protein concentrates in the bottom phase. A separation scheme consisting of two consecutive aqueous two-phase extraction steps was proposed: a first extraction in polyethylene glycol (PEG8000)–polyvinyl alcohol system, followed by a second extraction in PEG8000–(NH4)2SO4 system. This allowed the separation of endo-PG from polymer and the recycling of PEG polymer, since endo-PG was very strongly partitioned into the bottom phase of the PEG8000–(NH4)2SO4 system. Laboratory-scale experiments were performed to test the efficiency of this scheme. It was found that enzyme recovery was up to 91% with a total purification factor of about 1.9 and a concentration factor of more than 5. About 90% of the total PEG added into the systems can be recovered, and no reduction was obtained in the purification factor using recycled PEG. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI. | por |
dc.description.sponsorship | CEC (INCO-DC) - contract number ERB IC18 CT97 0182. | por |
dc.language.iso | eng | eng |
dc.publisher | Elsevier 1 | eng |
dc.rights | openAccess | eng |
dc.subject | Partitioning | eng |
dc.subject | Aqueous two-phase systems | eng |
dc.subject | Enzymes | eng |
dc.subject | Endo-polygalacturonase | eng |
dc.title | Separation of endo-polygalacturonase using aqueous two-phase partitioning | eng |
dc.type | article | eng |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 23 | por |
oaire.citationEndPage | 29 | por |
oaire.citationIssue | 1-2 | por |
oaire.citationVolume | 929 | por |
dc.identifier.doi | 10.1016/S0021-9673(01)01161-X | por |
dc.identifier.pmid | 11594400 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Chromatography A | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2001-33[1].pdf | 261,5 kB | Adobe PDF | Ver/Abrir |