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https://hdl.handle.net/1822/1609
Título: | Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3-phosphate dehydrogenase |
Autor(es): | Almeida, Catarina Queirós, Odília Wheals, Alan Teixeira, J. A. Ferreira, Pedro Moradas |
Palavras-chave: | Flocculation Glyceraldehyde-3-phosphate dehydrogenase Kluyveromyces |
Data: | 2003 |
Editora: | Elsevier 1 |
Revista: | Journal of Microbiological Methods |
Citação: | “Journal of microbiological methods”. ISSN 0167-7012. 55:2 (2003) 433-440. |
Resumo(s): | The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase–p37—that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engineering Kluyveromyces cells to display a flocculation phenotype, K. marxianus CCT 3172 was transformed with an expression vector containing GAP1. This vector is based on the pY37 previously described, harbouring a S11 Kluyveromyces origin of replication, and the expression of GAP1 is under the control of GAL1. Kluyveromyces cells overexpressing GAP1 acquired a flocculent phenotype together with the accumulation of p37 in the cell wall. The results support the use of GAP1 gene as a molecular tool for inducing flocculation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1609 |
DOI: | 10.1016/S0167-7012(03)00189-1 |
ISSN: | 0167-7012 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2003-02.pdf | 366,71 kB | Adobe PDF | Ver/Abrir |