Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1609
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Almeida, Catarina | - |
dc.contributor.author | Queirós, Odília | - |
dc.contributor.author | Wheals, Alan | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Ferreira, Pedro Moradas | - |
dc.date.accessioned | 2005-05-16T16:32:03Z | - |
dc.date.available | 2005-05-16T16:32:03Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | “Journal of microbiological methods”. ISSN 0167-7012. 55:2 (2003) 433-440. | eng |
dc.identifier.issn | 0167-7012 | - |
dc.identifier.uri | https://hdl.handle.net/1822/1609 | - |
dc.description.abstract | The use of flocculating yeast strains has been considered as a convenient approach to obtain high cell densities in bioreactors with increasing productivity in continuous operations. In Kluyveromyces marxianus ATTC 10022, the GAP1 gene encodes an isoform of glyceraldehyde-3-phosphate dehydrogenase–p37—that is accumulated in the cell wall and is involved in flocculation. To test the use of p37 as a tool for engineering Kluyveromyces cells to display a flocculation phenotype, K. marxianus CCT 3172 was transformed with an expression vector containing GAP1. This vector is based on the pY37 previously described, harbouring a S11 Kluyveromyces origin of replication, and the expression of GAP1 is under the control of GAL1. Kluyveromyces cells overexpressing GAP1 acquired a flocculent phenotype together with the accumulation of p37 in the cell wall. The results support the use of GAP1 gene as a molecular tool for inducing flocculation. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) - BD/18203/98. | por |
dc.language.iso | eng | eng |
dc.publisher | Elsevier 1 | eng |
dc.rights | openAccess | eng |
dc.subject | Flocculation | eng |
dc.subject | Glyceraldehyde-3-phosphate dehydrogenase | eng |
dc.subject | Kluyveromyces | eng |
dc.title | Acquisition of flocculation phenotype by Kluyveromyces marxianus when overexpressing GAP1 gene encoding an isoform of glyceraldehyde-3-phosphate dehydrogenase | eng |
dc.type | article | eng |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 433 | por |
oaire.citationEndPage | 440 | por |
oaire.citationIssue | 2 | por |
oaire.citationVolume | 55 | por |
dc.identifier.doi | 10.1016/S0167-7012(03)00189-1 | por |
dc.identifier.pmid | 14529965 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Microbiological Methods | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2003-02.pdf | 366,71 kB | Adobe PDF | Ver/Abrir |