Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1368
Título: | Methods to extract the exopolymeric matrix from biofilms : a comparative study |
Autor(es): | Azeredo, Joana Lazarova, V. Oliveira, Rosário |
Palavras-chave: | Biofilm Polymeric matrix Extraction methods Glutaraldehyde Cell leakage Exopolymers Proteins |
Data: | 1999 |
Editora: | Elsevier Science |
Revista: | Water Science and Technology |
Citação: | "Water science & technology". ISSN 0273-1223. 39:7 (1999) 243-250. |
Relatório da Série N.º: | 1999;03 |
Resumo(s): | To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization and the biofilm matrix is solubilized in the solution. |
Tipo: | Artigo em ata de conferência |
URI: | https://hdl.handle.net/1822/1368 |
DOI: | 10.1016/S0273-1223(99)00174-2 |
ISSN: | 0273-1223 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1999-03[1].pdf | 440,32 kB | Adobe PDF | Ver/Abrir |