Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1368
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Azeredo, Joana | - |
dc.contributor.author | Lazarova, V. | - |
dc.contributor.author | Oliveira, Rosário | - |
dc.date.accessioned | 2005-05-02T14:02:17Z | - |
dc.date.available | 2005-05-02T14:02:17Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | "Water science & technology". ISSN 0273-1223. 39:7 (1999) 243-250. | eng |
dc.identifier.issn | 0273-1223 | por |
dc.identifier.uri | https://hdl.handle.net/1822/1368 | - |
dc.description.abstract | To study the composition of a biofilm a previous extraction method is required to separate cells from the matrix. There are several methods reported in the literature; however they are not efficient or promote leakage of intracellular material. In this work several extraction methods were assayed in mixed culture and pure culture biofilms and their efficiency was evaluated by the amount of organic carbon, proteins and intracellular material extracted. The results showed that the extraction with glutaraldehyde 3% (w/v) was the most suitable method, extracting great amounts of organic carbon without promoting cell lysis or permeabilization and the biofilm matrix is solubilized in the solution. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI - PRAXIS/2/2.1BIO/37/94. | por |
dc.description.sponsorship | BIOTOBIO - TMR. | por |
dc.language.iso | eng | eng |
dc.publisher | Elsevier Science | eng |
dc.relation.ispartofseries | 1999;03 | eng |
dc.rights | openAccess | eng |
dc.subject | Biofilm | eng |
dc.subject | Polymeric matrix | eng |
dc.subject | Extraction methods | eng |
dc.subject | Glutaraldehyde | eng |
dc.subject | Cell leakage | eng |
dc.subject | Exopolymers | eng |
dc.subject | Proteins | eng |
dc.title | Methods to extract the exopolymeric matrix from biofilms : a comparative study | eng |
dc.type | conferencePaper | eng |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 243 | por |
oaire.citationEndPage | 250 | por |
oaire.citationIssue | 7 | por |
oaire.citationVolume | 39 | por |
dc.identifier.doi | 10.1016/S0273-1223(99)00174-2 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Water Science and Technology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1999-03[1].pdf | 440,32 kB | Adobe PDF | Ver/Abrir |