Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1086
Título: | The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei |
Autor(es): | Lemos, M. A. Teixeira, J. A. Domingues, M. R. M. Mota, M. Gama, F. M. |
Palavras-chave: | Cellulose binding domains Synergism Cellulases |
Data: | 2003 |
Editora: | Elsevier 1 |
Revista: | Enzyme and Microbial Technology |
Citação: | "Enzyme and microbial technology". ISSN 0141-0229. 32:1 (2003) 35-40. |
Relatório da Série N.º: | 2003;19 |
Resumo(s): | The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/1086 |
DOI: | 10.1016/S0141-0229(02)00235-1 |
ISSN: | 0141-0229 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
2003-19.pdf | 132,92 kB | Adobe PDF | Ver/Abrir |