Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/1086
Registo completo
Campo DC | Valor | Idioma |
---|---|---|
dc.contributor.author | Lemos, M. A. | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Domingues, M. R. M. | - |
dc.contributor.author | Mota, M. | - |
dc.contributor.author | Gama, F. M. | - |
dc.date.accessioned | 2005-04-15T14:13:53Z | - |
dc.date.available | 2005-04-15T14:13:53Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | "Enzyme and microbial technology". ISSN 0141-0229. 32:1 (2003) 35-40. | eng |
dc.identifier.issn | 0141-0229 | por |
dc.identifier.uri | https://hdl.handle.net/1822/1086 | - |
dc.description.abstract | The effect of isolated cellulose binding domains (CBDs) on the hydrolysis of filter paper and microcrystalline cellulose by both cellobiohydrolase I and endoglucanase, was studied. CBDs were obtained by proteolysis from cellulases using a scaled-up variant of our previous method. Experiments were performed for different enzyme/substrate ratios in both the absence and presence of CBDs. Hydrolysis of filter paper by intact cellobiohydrolase I in the presence of additional CBDs was found to have a synergistic effect, leading to an increase of the sugar production of up to 30%. The effect was less pronounced using microcrystalline cellulose, where an increase up to 16% was observed. Similar trends were found during the hydrolysis of both substrates by endoglucanase. | eng |
dc.description.sponsorship | Fundação para a Ciência e a Tecnologia (FCT) – PRAXIS XXI. | por |
dc.language.iso | eng | - |
dc.publisher | Elsevier 1 | eng |
dc.relation.ispartofseries | 2003;19 | - |
dc.rights | openAccess | eng |
dc.subject | Cellulose binding domains | eng |
dc.subject | Synergism | eng |
dc.subject | Cellulases | eng |
dc.title | The enhancement of the cellulolytic activity of cellobiohydrolase I and endoglucanase by the addition of cellulose binding domains derived from trichoderma reesei | eng |
dc.type | article | - |
dc.peerreviewed | yes | eng |
oaire.citationStartPage | 35 | por |
oaire.citationEndPage | 40 | por |
oaire.citationIssue | 1 | por |
oaire.citationVolume | 32 | por |
dc.identifier.doi | 10.1016/S0141-0229(02)00235-1 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Enzyme and Microbial Technology | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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2003-19.pdf | 132,92 kB | Adobe PDF | Ver/Abrir |