Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/10689
Título: | Identification of cell-surface mannans in a virulent Helicobacter pylori strain |
Autor(es): | Ferreira, José Alexandre Azevedo, N. F. Vieira, M. J. Figueiredo, C. Goodfellow, Brian J. Monteiro, Mário A. Coimbra, Manuel A. |
Palavras-chave: | Cell-surface glycans Helicobacter pylori Lipopolysaccharide Mannan Vaccine |
Data: | Abr-2010 |
Editora: | Elsevier 1 |
Revista: | Carbohydrate Research |
Citação: | Ferreira, J. A., Azevedo, N. F., Vieira, M. J., Figueiredo, C., Goodfellow, B. J., Monteiro, M. A., & Coimbra, M. A. (2010). Identification of cell-surface mannans in a virulent Helicobacter pylori strain. Carbohydrate Research, 345(6), 830-838. doi: https://doi.org/10.1016/j.carres.2010.01.022 |
Resumo(s): | With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations: (image) In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host–microbial interactions and immunological modulation. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/10689 |
DOI: | 10.1016/j.carres.2010.01.022 |
ISSN: | 0008-6215 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0008621510000467 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Ferreira_Carbohydrate Research.pdf | 612,04 kB | Adobe PDF | Ver/Abrir |