Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/10689
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Ferreira, José Alexandre | - |
dc.contributor.author | Azevedo, N. F. | - |
dc.contributor.author | Vieira, M. J. | - |
dc.contributor.author | Figueiredo, C. | - |
dc.contributor.author | Goodfellow, Brian J. | - |
dc.contributor.author | Monteiro, Mário A. | - |
dc.contributor.author | Coimbra, Manuel A. | - |
dc.date.accessioned | 2010-07-07T13:29:08Z | - |
dc.date.available | 2010-07-07T13:29:08Z | - |
dc.date.issued | 2010-04 | - |
dc.identifier.citation | Ferreira, J. A., Azevedo, N. F., Vieira, M. J., Figueiredo, C., Goodfellow, B. J., Monteiro, M. A., & Coimbra, M. A. (2010). Identification of cell-surface mannans in a virulent Helicobacter pylori strain. Carbohydrate Research, 345(6), 830-838. doi: https://doi.org/10.1016/j.carres.2010.01.022 | por |
dc.identifier.issn | 0008-6215 | por |
dc.identifier.uri | https://hdl.handle.net/1822/10689 | - |
dc.description.abstract | With the intent of contributing to a carbohydrate-based vaccine against the gastroduodenal pathogen, Helicobacter pylori, we report here the structure of cell-surface mannans obtained from a virulent strain. Unlike other wild-type strains, this strain was found to express in good quantities this polysaccharide in vitro. Structural analysis revealed a branched mannan formed by a backbone of α-(1→6)-linked mannopyranosyl residues with approximately 80% branching at the O-2 position. The branches were composed of O-2-linked Man residues in both α- and β-configurations: (image) In addition, this strain also expressed cell-surface emblematic H. pylori lipopolysaccharides (LPS) containing partially fucosylated polyLacNAc O-chains. Affinity assays with polymyxin-B and concanavalin A revealed no association between the mannan and the LPS. The described mannans may be implicated in the mediation of host–microbial interactions and immunological modulation. | por |
dc.description.sponsorship | This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) through project Pylori E&LPS POCI/QUI/56393/2004, PhD grant SFRH/BD/19929/2004, by the Natural Sciences and Engineering Research Council of Canada (NSERC), and by the European Network of Research Infrastructures within the 6th Framework Programme of the EC (Contract # RII3-026145, EU-NMR). The authors further thank Dr. Adrien Favier (RALF-NMR facility, Grenoble - France) for conducting NMR experiments. | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier 1 | por |
dc.relation | info:eu-repo/grantAgreement/FCT/POCI/56393/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBD%2F19929%2F2004/PT | - |
dc.rights | openAccess | por |
dc.subject | Cell-surface glycans | por |
dc.subject | Helicobacter pylori | por |
dc.subject | Lipopolysaccharide | por |
dc.subject | Mannan | por |
dc.subject | Vaccine | por |
dc.title | Identification of cell-surface mannans in a virulent Helicobacter pylori strain | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.sciencedirect.com/science/article/pii/S0008621510000467 | - |
sdum.number | 6 | por |
sdum.pagination | 830–838 | por |
sdum.publicationstatus | published | por |
sdum.volume | 345 | por |
oaire.citationStartPage | 830 | por |
oaire.citationEndPage | 838 | por |
oaire.citationIssue | 6 | por |
oaire.citationVolume | 345 | por |
dc.identifier.doi | 10.1016/j.carres.2010.01.022 | por |
dc.identifier.pmid | 20227685 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Carbohydrate Research | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Ferreira_Carbohydrate Research.pdf | 612,04 kB | Adobe PDF | Ver/Abrir |