Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/9637
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Machado, Raul | - |
dc.contributor.author | Ribeiro, Artur J. | - |
dc.contributor.author | Padrão, Jorge | - |
dc.contributor.author | Silva, D. | - |
dc.contributor.author | Nobre, Alexandra | - |
dc.contributor.author | Teixeira, J. A. | - |
dc.contributor.author | Arias, F. J. | - |
dc.contributor.author | Cunha, A. M. | - |
dc.contributor.author | Rodríguez-Cabello, José Carlos | - |
dc.contributor.author | Casal, Margarida | - |
dc.date.accessioned | 2009-10-01T14:13:44Z | - |
dc.date.available | 2009-10-01T14:13:44Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Machado, R., Ribeiro, A. J., Padrão, J., Silva, D., Nobre, A., Teixeira, J. A., … Casal, M. (2009, June). Exploiting the Sequence of Naturally Occurring Elastin: Construction, Production and Characterization of a Recombinant Thermoplastic Protein-Based Polymer. Journal of Nano Research. Trans Tech Publications, Ltd. http://doi.org/10.4028/www.scientific.net/jnanor.6.133 | por |
dc.identifier.issn | 1662-5250 | por |
dc.identifier.uri | https://hdl.handle.net/1822/9637 | - |
dc.description.abstract | Genetic engineering was used to produce an elastin-like polymer (ELP) with precise amino acid composition, sequence and length, resulting in the absolute control of MW and stereochemistry. A synthetic monomer DNA sequence encoding for (VPAVG)20, was used to build a library of concatemer genes with precise control on sequence and size. The higher molecular weight polymer with 220 repeats of VPAVG was biologically produced in Escherichia coli and purified by hot and cold centrifugation cycles, based on the reversible inverse temperature transition property of ELPs. The use of low cost carbon sources like lactose and glycerol for bacteria cells culture media was explored using Central Composite Design approach allowing optimization of fermentation conditions. Due to its self-assembling behaviour near 33 oC stable spherical microparticles with a size ~ 1μm were obtained, redissolving when a strong undercooling is achieved. The polymer produced showed hysteresis behaviour with thermal absorbing/releasing components depending on the salt concentration of the polymer solution. | eng |
dc.description.sponsorship | This work was supported by the ElastM Project, POCI/CTM/57177/2004 from Fundação para a Ciência e Tecnologia (Portugal) and FEDER, “Junta de Castilla y Leon” (VA087A06, VA016B08 and VA030A08), MEC (MAT2007-66275-C02-01 and NAN2004-08538), and by the Marie Curie RTN Biopolysurf (MRTN-CN-2004-005516). Raul Machado received a PhD grant: SFRH/BD/36754/2007 from Fundação para a Ciência e Tecnologia (Portugal). The authors would like to thank Magda Graça (CBMA) for all the technical support in sequencing, Lígia Rodrigues for the CCD enlightenment and Dan W. Urry for the stimulating discussions and encouragement to pursue the studies on protein-based polymers. | por |
dc.language.iso | eng | por |
dc.publisher | Trans Tech Publications | por |
dc.relation | info:eu-repo/grantAgreement/FCT/FARH/SFRH%2FBD%2F36754%2F2007/PT | - |
dc.relation | info:eu-repo/grantAgreement/FCT/POCI/POCI%2FCTM%2F57177%2F2004/PT | - |
dc.rights | openAccess | por |
dc.subject | Elastin-like polymers | por |
dc.subject | VPAVG | por |
dc.subject | Nanoparticles | por |
dc.subject | Thermoplastic | por |
dc.subject | Thermoresponsive polymer | por |
dc.title | Exploiting the sequence of naturally occurring elastin: construction, production and characterization of a recombinant thermoplastic protein-based polymer | eng |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.scientific.net/JNanoR.6.133 | por |
sdum.pagination | 133-145 | por |
sdum.publicationstatus | published | por |
sdum.volume | 6 | por |
oaire.citationStartPage | 133 | por |
oaire.citationEndPage | 145 | por |
oaire.citationVolume | 6 | por |
dc.identifier.eissn | 1661-9897 | - |
dc.identifier.doi | 10.4028/www.scientific.net/JNanoR.6.133 | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | Journal of Nano Research | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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JNanoR.6.133.pdf | Documento principal | 796,38 kB | Adobe PDF | Ver/Abrir |